Felkner I C
Mutat Res. 1977 Feb;42(2):175-80. doi: 10.1016/s0027-5107(77)80021-3.
A procedure was developed to select for specific mutations obtained by means of transformation with DNA previously exposed to potentially dangerous chemical compounds. The 70% co-transformation of hisB and trpC genes in Bacillus subtilis provided a convenient opportunity to select for new mutations. When purified DNA from wild type bacteria was treated with N(OH) acetyl aminofluorene or Hoechst dye 37 507 and used to transform a recipient bearing of a trpC2 mutaion, a high proportion of the Trp+ transformants had new hisB mutations.
已开发出一种程序，用于筛选通过用先前暴露于潜在危险化合物的DNA进行转化而获得的特定突变。枯草芽孢杆菌中hisB和trpC基因70%的共转化为筛选新突变提供了便利机会。当用N（OH）乙酰氨基芴或赫斯特染料37507处理来自野生型细菌的纯化DNA，并用于转化携带trpC2突变的受体时，很大比例的Trp+转化体具有新的hisB突变。
