用氮芥交联DNA对枯草芽孢杆菌进行转化。对共转化和突变频率的影响。

Transformation in Bacillus subtilis with nitrogen mustard crosslinked DNA. Effect on cotransformation and mutation frequencies.

作者信息

Scheinbach S, Rudner R

出版信息

Biochim Biophys Acta. 1978 Dec 21;521(2):484-92. doi: 10.1016/0005-2787(78)90290-3.

Abstract

Bacillus subtilis DNA was treated in vitro with nitrogen mustard and the crosslinked molecules were purified, after alkali denaturation, by hydroxyapatite chromatography. When tested for the ability to transform the trpC2-hisB2 segment, these molecules exhibited a decrease in the cotransformation index (r) as compared to native or renatured DNA. The decrease in r was not accompanied by an increase in mutagenicity.

摘要

用氮芥对枯草芽孢杆菌DNA进行体外处理，交联分子经碱变性后通过羟基磷灰石柱层析进行纯化。当检测这些分子转化trpC2-hisB2片段的能力时，与天然或复性DNA相比，它们的共转化指数（r）有所降低。r值的降低并未伴随着致突变性的增加。