Rivera Lautaro, Popov Pereira da Cunha Matthäus Davi, Sabbatella Rocío Jazmín, Del Veliz Samanta, Abraham Gustavo Abel, Uhart Marina, Aldana Ana Agustina
Instituto de Histología y Embriología de Mendoza (IHEM, CONICET-Universidad Nacional de Cuyo), Mendoza, Argentina.
Instituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA, CONICET-Universidad Nacional de Mar del Plata), Mar del Plata, Argentina.
J Biomed Mater Res A. 2025 May;113(5):e37914. doi: 10.1002/jbm.a.37914.
Drawing inspiration from the extracellular matrix (ECM), where fiber organization profoundly influences cell behavior, electrospun scaffolds have emerged as powerful tools for modulating cellular responses in vitro. While electrospinning enables the replication of ECM architecture, selecting suitable materials is paramount for effective cell adhesion and growth. In this study, we aimed to develop cost-effective scaffolds for human adipose-derived mesenchymal stromal cells (hAD-MSCs) using gelatin methacrylate (GelMA) blended with either polycaprolactone (PCL) or poly(ethylene glycol) dimethacrylate (PEGDMA). Through comparing randomly oriented and aligned fibers, we identified fiber direction as a critical factor in determining cell behavior. Surprisingly, we found that despite material hydrophobicity, the cells aligned with the fiber direction, highlighting the dominant influence of fiber alignment on cell spreading. This research underscores the importance of material selection and fiber orientation in engineering scaffolds for directing cell behavior in tissue regeneration applications.
受细胞外基质（ECM）的启发，其中纤维组织深刻影响细胞行为，电纺支架已成为体外调节细胞反应的有力工具。虽然电纺能够复制ECM结构，但选择合适的材料对于有效的细胞粘附和生长至关重要。在本研究中，我们旨在使用与聚己内酯（PCL）或聚（乙二醇）二甲基丙烯酸酯（PEGDMA）混合的甲基丙烯酸明胶（GelMA）开发用于人脂肪来源间充质基质细胞（hAD-MSCs）的经济高效的支架。通过比较随机取向和排列的纤维，我们确定纤维方向是决定细胞行为的关键因素。令人惊讶的是，我们发现尽管材料具有疏水性，但细胞仍与纤维方向对齐，突出了纤维排列对细胞铺展的主要影响。这项研究强调了材料选择和纤维取向在工程支架中指导组织再生应用中细胞行为的重要性。
