Wang H Z, Donaldson A, Sufi S B, Jeffcoate S L
Ann Clin Biochem. 1985 May;22 ( Pt 3):316-20. doi: 10.1177/000456328502200316.
Prolactin has been reported to be present in cervical mucus at concentrations higher than those found in blood. Our initial findings appeared to confirm this and the material fulfilled criteria of validity generally applied when an immunoassay is employed on a new biological matrix, i.e. parallelism and chromatographic identity. Further experiments demonstrated that prolactin concentrations in cervical mucus were less than 40 mU/L and the prolactin-like immunoreactivity originally detected was due to the action of the enzyme bromelin which was used to liquefy the mucus. Bromelin has a similar molecular weight to prolactin and appeared to digest prolactin tracer and reduce its ability to bind antiserum in a manner paralleling the effect of adding pituitary prolactin.
据报道，催乳素在宫颈黏液中的浓度高于其在血液中的浓度。我们最初的研究结果似乎证实了这一点，并且该物质符合在新生物基质上进行免疫测定时通常应用的有效性标准，即平行性和色谱同一性。进一步的实验表明，宫颈黏液中的催乳素浓度低于40 mU/L，最初检测到的催乳素样免疫反应性是由于用于液化黏液的菠萝蛋白酶的作用。菠萝蛋白酶的分子量与催乳素相似，并且似乎以与添加垂体催乳素的作用平行的方式消化催乳素示踪剂并降低其与抗血清结合的能力。
