Ma Hongyi, Li Chengnan, Li Shan, Zhao Yulei, Ma Wenling, Wang Rui, Guo Ningning, Yao Wenkong, Yin Xiao
College of Enology and Horticulture, Ningxia University, Yinchuan 750021, China.
J Agric Food Chem. 2025 May 7;73(18):11435-11451. doi: 10.1021/acs.jafc.4c12810. Epub 2025 Apr 22.
The fruit peel serves as the frontline defense of grapes against pathogens like , yet its defense mechanisms remain poorly understood. This study reveals novel resistance mechanisms underlying peel immunity through comparative transcriptomic and metabolomic analysis of "Bei Binghong" (BH) and "Red Globe" (RG). The analysis identified 1277 differentially expressed genes (DEGs) and 38 differentially accumulated metabolites (DAMs), primarily associated with secondary metabolic processes and plant hormone signaling pathways. Weighted gene coexpression network analysis (WGCNA) uncovered three key modules and several novel hub genes. Crucially, transcriptomic profiling identified as a central regulator. Postinfection, upregulated genes and metabolites were involved in salicylic acid (SA), lignin, and stilbene biosynthesis in BH, as well as enhanced resistance through overexpression of . A conceptual model for defense against was proposed, providing novel insights into grapevine defense mechanisms.
果皮是葡萄抵御诸如[病原体名称未给出]等病原体的第一道防线，但其防御机制仍知之甚少。本研究通过对“北冰红”（BH）和“红地球”（RG）进行比较转录组学和代谢组学分析，揭示了果皮免疫背后的新抗性机制。分析确定了1277个差异表达基因（DEG）和38种差异积累代谢物（DAM），主要与次生代谢过程和植物激素信号通路相关。加权基因共表达网络分析（WGCNA）发现了三个关键模块和几个新的枢纽基因。至关重要的是，转录组分析确定[关键调控因子未给出]为核心调节因子。感染后，BH中上调的基因和代谢物参与水杨酸（SA）、木质素和芪的生物合成，并通过[基因未给出]的过表达增强抗性。提出了一个针对[病原体未给出]的防御概念模型，为葡萄防御机制提供了新见解。
