Wu Bohong, Zhou Yuan, Jia Xueying, Wang Congyi, Chen Lijie, Fan Haiyan, Wang Yuanyuan, Liu Xiaoyu, Yang Ning, Duan Yuxi, Xuan Yuanhu, Zhu Xiaofeng
Nematology Institute of Northern China, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, China.
Phytopathology. 2025 Jul;115(7):881-890. doi: 10.1094/PHYTO-02-25-0059-R. Epub 2025 Jul 15.
SWEET (Sugars Will Eventually be Exported Transporter) proteins facilitate the movement of sugars through cell membranes and are essential for loading sucrose into phloem. Beyond its role in sugar transport, the SWEET protein also modulates plant resistance to various biotic and abiotic stresses. Among sugar transporter genes, is a positive regulatory factor involved in soybean cyst nematode () resistance. In this study, susceptible soybean cultivars (Williams 82) were used to conduct a transcriptome analysis to characterize the responses to nematode infection, in which multiple sugar transporter genes were highly expressed. The RT-qPCR analysis confirmed a significant increase in the expression of in soybean roots with infection. Heterologous expression tests indicated that the protein encoded by does not transport hexose in yeast. Further analysis showed that soybean lines overexpressing exhibited increased resistance to compared with the control. A yeast one-hybrid assay was employed to discover that LOC114390362 binds to the promoter. Transient expression in tobacco leaf cells revealed nucleus and cytosolic localization of LOC114390362. -overexpressing soybean lines showed a reduced number of nematode infections. Overall, the results indicate that the binding of LOC114390362 to the promoter plays a positive role in regulating soybean resistance to . The gene has great potential to improve resistance to plant-parasitic nematodes in soybean and other plants.
SWEET（糖最终输出转运蛋白）蛋白促进糖类通过细胞膜的转运，对于将蔗糖装载到韧皮部至关重要。除了在糖转运中的作用外，SWEET蛋白还调节植物对各种生物和非生物胁迫的抗性。在糖转运蛋白基因中， 是参与大豆胞囊线虫（）抗性的正调控因子。在本研究中，使用感病大豆品种（Williams 82）进行转录组分析，以表征对线虫感染的反应，其中多个糖转运蛋白基因高度表达。RT-qPCR分析证实，在 感染的大豆根中， 的表达显著增加。异源表达试验表明， 编码的蛋白在酵母中不转运己糖。进一步分析表明，与对照相比，过表达 的大豆品系对 表现出增强的抗性。采用酵母单杂交试验发现LOC114390362与 启动子结合。在烟草叶细胞中的瞬时表达揭示了LOCl14390362的细胞核和细胞质定位。过表达 的大豆品系显示线虫感染数量减少。总体而言，结果表明LOC114390362与 启动子的结合在调节大豆对 的抗性中起积极作用。 基因在提高大豆和其他植物对植物寄生线虫的抗性方面具有巨大潜力。
