Zhang Zheng, Li Qiuling, Fan Xing, You Gan
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Department of Neurophysiology, Beijing Neurosurgical Institute, Capital Medical University, Beijing, 100070, China.
Sci Rep. 2025 Apr 22;15(1):13911. doi: 10.1038/s41598-025-97855-x.
Epilepsy is often the initial symptom in two-thirds of glioblastoma (GBM) patients. Existing studies have shown that microRNAs (miRNAs) play a crucial role in epilepsy. However, their role in epilepsy associated with glioblastoma remains unclear. The aim of our study was to investigate the correlation between miR-1290 expression in GBM patients and pre-operative seizures, as well as patient outcomes. 81 GBM patients were enrolled in our study, and an independent validation was carried out with 92 similar cases. MiRNA profiling of the 81 patients was conducted to identify differentially expressed miRNAs. In the validation cohort, key miRNAs were validated by using quantitative reverse transcriptase polymerase chain reaction (q-PCR). Additionally, functional analysis of these miRNAs was performed through Gene Ontology (GO) analysis. Our array analysis disclosed that there were seven under-expressed miRNAs in patients with preoperative seizures when compared to those without preoperative seizures. Among them, miR-1290 showed the highest fold change. Validation in an independent cohort verified that patients with favorable seizure outcomes had higher miR-1290 expression levels. Functional enrichment analysis demonstrated that the gene expression profiles associated with miR-1290 were enriched in biological processes related to transcription and cell cycle regulation, especially the functions mediated by RNA polymerase II. MiR-1290 emerges as a promising biomarker for predicting seizure susceptibility and overall survival in GBM patients. A specific evaluation of miR-1290 may lead to targeted diagnostic and therapeutic interventions, potentially providing novel strategies for enhancing patient outcomes.
癫痫常是三分之二胶质母细胞瘤（GBM）患者的首发症状。现有研究表明，微小RNA（miRNA）在癫痫中起关键作用。然而，它们在与胶质母细胞瘤相关的癫痫中的作用仍不清楚。我们研究的目的是调查GBM患者中miR-1290表达与术前癫痫发作以及患者预后之间的相关性。81例GBM患者纳入我们的研究，并对92例类似病例进行了独立验证。对81例患者进行miRNA谱分析以鉴定差异表达的miRNA。在验证队列中，通过定量逆转录聚合酶链反应（q-PCR）对关键miRNA进行验证。此外，通过基因本体论（GO）分析对这些miRNA进行功能分析。我们的阵列分析显示，与无术前癫痫发作的患者相比，有术前癫痫发作的患者中有7种miRNA表达下调。其中，miR-1290的变化倍数最高。独立队列中的验证证实，癫痫发作预后良好的患者miR-1290表达水平较高。功能富集分析表明，与miR-1290相关的基因表达谱在与转录和细胞周期调控相关的生物学过程中富集，特别是由RNA聚合酶II介导的功能。miR-1290有望成为预测GBM患者癫痫易感性和总生存期的生物标志物。对miR-1290的特异性评估可能会导致有针对性的诊断和治疗干预，有可能为改善患者预后提供新策略。
