Xie Yuxiao, Liu Jingjing, Ma Jun, Shi Nan, Zhang Xiumin
College of Life Sciences, Hebei University, Baoding, China.
Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding, China.
Front Microbiol. 2025 Apr 8;16:1557511. doi: 10.3389/fmicb.2025.1557511. eCollection 2025.
This study is aimed at the actual demand for exploring new species resources of , and aims to solve the technical bottleneck of isolation and culture. A new method was established based on the resuscitation function of the RpfA protein from CGMCC 4.1658 to isolate unculturable or difficult-to-culture species, and it was applied to explore species resources in special habitats in the frozen soils of the Qinghai-Tibet Plateau. The RpfA protein of was heterologously expressed and validated for its activity. The purified RpfA protein was then used to isolate from soil samples in the frozen soils of the Qinghai-Tibet Plateau, followed by an investigation into the impact of the RpfA protein on the cultivability of species. The results showed that the RpfA protein had a significant promoting effect on the germination of spores of both itself and other species of the genus, and when a suitable concentration of RpfA protein was added to the culture medium, it could significantly improve the culturability of members of phylum , especially species. In addition, many new species of the genus and other genera of phylum were discovered. This study provides a new approach for further exploring species resources in special environments such as the Qinghai-Tibet Plateau and developing new biologically active substances produced by .
本研究针对探索[具体物种]新物种资源的实际需求，旨在解决[具体物种]分离培养的技术瓶颈。基于来自中国普通微生物菌种保藏管理中心4.1658的RpfA蛋白的复苏功能建立了一种新方法，用于分离不可培养或难培养的[具体物种]，并将其应用于探索青藏高原冻土特殊生境中的[具体物种]资源。对[具体物种]的RpfA蛋白进行了异源表达并验证其活性。然后用纯化的RpfA蛋白从青藏高原冻土土壤样品中分离[具体物种]，接着研究RpfA蛋白对[具体物种]可培养性的影响。结果表明，RpfA蛋白对其自身及[具体属]其他物种的孢子萌发均有显著促进作用，当在培养基中添加合适浓度的RpfA蛋白时，可显著提高[具体门]成员尤其是[具体物种] 的可培养性。此外，还发现了[具体属]的许多新物种以及[具体门]的其他属。本研究为进一步探索青藏高原等特殊环境中的[具体物种]资源以及开发[具体物种]产生的新型生物活性物质提供了新途径。
