Excavation of resources of species in frozen soils of the Qinghai-Tibet Plateau based on RpfA protein of .

This study is aimed at the actual demand for exploring new species resources of , and aims to solve the technical bottleneck of isolation and culture. A new method was established based on the resuscitation function of the RpfA protein from CGMCC 4.1658 to isolate unculturable or difficult-to-culture species, and it was applied to explore species resources in special habitats in the frozen soils of the Qinghai-Tibet Plateau. The RpfA protein of was heterologously expressed and validated for its activity. The purified RpfA protein was then used to isolate from soil samples in the frozen soils of the Qinghai-Tibet Plateau, followed by an investigation into the impact of the RpfA protein on the cultivability of species. The results showed that the RpfA protein had a significant promoting effect on the germination of spores of both itself and other species of the genus, and when a suitable concentration of RpfA protein was added to the culture medium, it could significantly improve the culturability of members of phylum , especially species. In addition, many new species of the genus and other genera of phylum were discovered. This study provides a new approach for further exploring species resources in special environments such as the Qinghai-Tibet Plateau and developing new biologically active substances produced by .