College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.
Institute of Agricultural Product Quality Standard and Testing Research, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, China.
Pol J Microbiol. 2021 Mar;70(1):87-97. doi: 10.33073/pjm-2021-008. Epub 2021 Mar 19.
The soil bacterial communities have been widely investigated. However, there has been little study of the bacteria in Qinghai-Tibet Plateau, especially about the culturable bacteria in highland barley cultivation soil. Here, a total of 830 individual strains were obtained at 4°C and 25°C from a highland barley cultivation soil in Qamdo, Tibet Autonomous Region, using fifteen kinds of media. Seventy-seven species were obtained, which belonged to 42 genera and four phyla; the predominant phylum was Actinobacteria (68.82%), followed by Proteobacteria (15.59%), Firmicutes (14.29%), and Bacteroidetes (1.30%). The predominant genus was (22.08%, 17 species), followed by (6.49%, five species), (5.19%, four species), (5.19%, four species), and (3.90%, three species). The most diverse isolates belonged to a high G+C Gram-positive group; in particular, the genus is a dominant genus in the high G+C Gram-positive group. There were 62 species and 33 genera bacteria isolated at 25°C (80.52%), 23 species, and 18 genera bacteria isolated at 4°C (29.87%). Meanwhile, only eight species and six genera bacteria could be isolated at 25°C and 4°C. Of the 77 species, six isolates related to six genera might be novel taxa. The results showed abundant bacterial species diversity in the soil sample from the Qamdo, Tibet Autonomous Region.
土壤细菌群落已被广泛研究。然而，对于青藏高原的细菌研究较少，特别是关于青稞种植土壤中的可培养细菌。在这里，从西藏自治区的察多的青稞种植土壤中，使用 15 种培养基，在 4°C 和 25°C 下共获得了 830 株单株。共获得了 77 个种，属于 42 个属和 4 个门；优势门是放线菌（68.82%），其次是变形菌门（15.59%）、厚壁菌门（14.29%）和拟杆菌门（1.30%）。优势属是（22.08%，17 种），其次是（6.49%，5 种）、（5.19%，4 种）、（5.19%，4 种）和（3.90%，3 种）。最具多样性的分离物属于高 G+C 阳性革兰氏菌组；特别是属是高 G+C 阳性革兰氏菌组中的优势属。在 25°C 下分离出 62 个种和 33 个属细菌（80.52%），在 4°C 下分离出 23 个种和 18 个属细菌（29.87%）。同时，只有 8 个种和 6 个属细菌可以在 25°C 和 4°C 下分离。在 77 个种中，有 6 个与 6 个属相关的分离物可能是新的分类单元。结果表明，来自西藏自治区察多的土壤样本中存在丰富的细菌物种多样性。
