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一种基于检测抗pEP153R、eGFP和p72蛋白抗体的新型非洲猪瘟病毒鉴别诊断（DIVA，区分感染动物和免疫动物）血清学检测方法。

A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 Proteins.

作者信息

González-García Gabriela, Gallardo Carmina, Montón Mercedes, Barroso-Arévalo Sandra, Casado Nadia, Barasona José Ángel, Sánchez-Vizcaíno José Manuel, Venteo Ángel, Sastre Patricia, Rueda Paloma

机构信息

Gold Standard Diagnostics Madrid SA (GSD Madrid), 28037 Madrid, Spain.

European Union Reference Laboratory for African Swine Fever (EURL), Centro de Investigación en Sanidad Animal (CISA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Científicas (CSIC), Valdeolmos, 28130 Madrid, Spain.

出版信息

Vaccines (Basel). 2025 Feb 20;13(3):211. doi: 10.3390/vaccines13030211.

DOI:10.3390/vaccines13030211
PMID:40266072
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11945662/
Abstract

BACKGROUND/OBJECTIVES: African Swine Fever (ASF) is one of the most significant infectious diseases affecting both domestic pig and wild boar populations, leading to substantial economic and biosanitary consequences. In Europe, disease management relies on stringent biosecurity measures and surveillance through diagnosis, highlighting the urgent need for an effective and safe vaccine for ASF control. In this context, the VACDIVA project has generated several promising vaccine candidates, including those with the EP153R gene deleted and replaced by the eGFP reporter gene.

METHODS

In this study, pEP153R and eGFP proteins were produced using recombinant technology and demonstrated their antigenicity and DIVA capability through indirect ELISA. Additionally, a prototype serological DIVA test was designed and developed. The assay is based on the detection of antibodies against both DIVA antigens and the well-established immunogenic p72 protein.

RESULTS

This preliminary DIVA diagnostic assay complements vaccine candidates based on a genotype II ASFV strain, featuring the deletion of the EP153R gene and/or the insertion of the eGFP reporter gene, exemplified by the Lv17/WB/Rie1-∆CD vaccine candidate.

CONCLUSIONS

This approach could potentially improve surveillance during prospective vaccination campaigns.

摘要

背景/目的：非洲猪瘟（ASF）是影响家猪和野猪种群的最重要的传染病之一，会导致重大的经济和生物安全后果。在欧洲，疾病管理依赖于严格的生物安全措施以及通过诊断进行监测，这凸显了迫切需要一种有效且安全的疫苗来控制非洲猪瘟。在此背景下，VACDIVA项目已研发出几种有前景的疫苗候选物，包括那些EP153R基因被删除并用eGFP报告基因替代的候选物。

方法

在本研究中，利用重组技术生产了pEP153R和eGFP蛋白，并通过间接ELISA证明了它们的抗原性和鉴别诊断能力。此外，设计并开发了一种原型血清学鉴别诊断检测方法。该检测基于对针对鉴别诊断抗原和成熟的免疫原性p72蛋白的抗体的检测。

结果

这种初步的鉴别诊断检测方法补充了基于基因型II非洲猪瘟病毒株的疫苗候选物，其特征为删除了EP153R基因和/或插入了eGFP报告基因，以Lv17/WB/Rie1-∆CD疫苗候选物为例。

结论

这种方法可能会改善前瞻性疫苗接种运动期间的监测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eead/11945662/7535be727854/vaccines-13-00211-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eead/11945662/34af44483ba8/vaccines-13-00211-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eead/11945662/6e083a0538c8/vaccines-13-00211-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eead/11945662/4c1d951bfd6f/vaccines-13-00211-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eead/11945662/f4aece13b95d/vaccines-13-00211-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eead/11945662/50c44bd730be/vaccines-13-00211-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eead/11945662/7535be727854/vaccines-13-00211-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eead/11945662/34af44483ba8/vaccines-13-00211-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eead/11945662/6e083a0538c8/vaccines-13-00211-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eead/11945662/4c1d951bfd6f/vaccines-13-00211-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eead/11945662/f4aece13b95d/vaccines-13-00211-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eead/11945662/50c44bd730be/vaccines-13-00211-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eead/11945662/7535be727854/vaccines-13-00211-g006.jpg

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2
Administration Routes and Doses of the Attenuated African Swine Fever Virus Strain PSA-1NH Influence Cross-Protection of Pigs against Heterologous Challenge.减毒非洲猪瘟病毒株PSA-1NH的给药途径和剂量影响猪对异源攻击的交叉保护作用。
Animals (Basel). 2024 Apr 24;14(9):1277. doi: 10.3390/ani14091277.
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The Production of Recombinant African Swine Fever Virus Lv17/WB/Rie1 Strains and Their In Vitro and In Vivo Characterizations.重组非洲猪瘟病毒Lv17/WB/Rie1株的产生及其体外和体内特性研究
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