猪纹状体膜中[3H]舒必利结合位点的表征。

The characterization of [3H]sulpiride binding sites in pig striatal membranes.

作者信息

Holden-Dye L, Poat J A, Senior K, Woodruff G N

出版信息

Biochem Pharmacol. 1985 Aug 15;34(16):2905-9. doi: 10.1016/0006-2952(85)90014-0.

Abstract

Pig striatal membranes have [3H]sulpiride-binding sites similar to those identified in rat striatal membranes. The pharmacological profile indicates that this binding is to dopamine receptors. Agonist displacement of [3H]sulpiride binding in pig striatal membranes is subject to guanine nucleotide regulation. This effect is mimicked by heat treatment. N-ethyl maleamide (20 microM) and dithioerythritol (3 mM) decrease agonist affinity for the [3H]sulpiride-binding site in pig striatal membranes without significantly affecting maximal displacement.

摘要

猪纹状体膜具有与大鼠纹状体膜中鉴定出的类似的[3H]舒必利结合位点。药理学特征表明这种结合是与多巴胺受体的结合。猪纹状体膜中[3H]舒必利结合的激动剂置换受鸟嘌呤核苷酸调节。这种效应可被热处理模拟。N-乙基马来酰胺（20微摩尔）和二硫苏糖醇（3毫摩尔）降低了猪纹状体膜中激动剂对[3H]舒必利结合位点的亲和力，而对最大置换没有显著影响。