Filice G, Carnevale G, Lanzarini P, Castelli F, Benzi-Cipelli R, Gorini G, Carosi G, Rondanelli E G
Boll Ist Sieroter Milan. 1985;64(2):119-25.
The AA. analyze the ultrastructural and immunological patterns of a low virulent strain of T. gondii cultured in vitro on A-9 cells line (PV-H1C-83). The tissue culture method described here is highly recommended to study the immunological aspects of experimental toxoplasmosis. Biological tests' results and the time courses of serological tests are also similar to those obtained from mice infected with cerebral tissue containing cysts. The low virulent strain (PV-H1C-83), when cultured in vitro, is able to induce the formation of large parasitophorous vacuoles, in which the EM investigation shows a typical cyst wall enveloping numerous bradyzoites.
作者分析了在A-9细胞系（PV-H1C-83）上体外培养的低毒力弓形虫株的超微结构和免疫模式。强烈推荐此处描述的组织培养方法用于研究实验性弓形虫病的免疫学方面。生物学测试结果和血清学测试的时间进程也与从感染含囊肿脑组织的小鼠获得的结果相似。低毒力株（PV-H1C-83）在体外培养时能够诱导形成大的寄生泡，电子显微镜观察显示典型的囊壁包裹着许多缓殖子。
