Sholl S A, Wolf R C, Colás A E
Steroids. 1977 Feb;29(2):237-48. doi: 10.1016/0039-128x(77)90042-3.
Cholesterol side-chain cleavage (CSCC) and aromatase activities were measured in luteal mitochondria and tissue pieces, respectively, from rhesus monkeys on days 22, 49, 128 and 160 of gestation. CSCC activity did not vary significantly during gestation and thus probably does not respond to chorionic gonadotropin which is elevated on day 22 of pregnancy. It is not known, however, whether CSCC can be stimulated prior to day 22 when the corpus luteum is steroidogenically more active. Both 3H-pregnenolone and 3H-progesterone were synthesized from [1,2-3/]cholesterol. Aromatase activity declined from high levels on days 22 and 49 to a nadir on day 128 of pregnancy. Utilizing either [1beta-3H]androstenedione or [1beta-3H]testosterone as substrate yielded comparable results throughout gestation.
分别在妊娠第22、49、128和160天，测定了恒河猴黄体线粒体和组织块中的胆固醇侧链裂解（CSCC）和芳香化酶活性。CSCC活性在妊娠期间无显著变化，因此可能对妊娠第22天升高的绒毛膜促性腺激素无反应。然而，尚不清楚在妊娠第22天之前，当黄体的类固醇生成活性更高时，CSCC是否能被刺激。3H-孕烯醇酮和3H-孕酮均由[1,2-3H]胆固醇合成。芳香化酶活性从妊娠第22天和第49天的高水平下降到第128天的最低点。在整个妊娠期，以[1β-3H]雄烯二酮或[1β-3H]睾酮为底物均得到了类似的结果。
