Zhang Lianjun, Zhuang Ziyan, Xie Jingang, Kong Wenting, Li Tingting, Wang Shengfei, Liu Xiaoning
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, National Demonstration Center for Experimental Biology Education, College of Life Science and Technology, Xinjiang University, Urumqi, China.
J Econ Entomol. 2025 Jun 13;118(3):1395-1406. doi: 10.1093/jee/toaf067.
Aphis gossypii Glover is a serious pest that causes significant economic losses and is difficult to control due to the rapid development of resistance to neonicotinoids. Insect cytochrome P450s play an important role in detoxification of insecticides. In this study, we aimed to understand the function of CYP380C12 in A. gossypii. The CYP380C12 gene of A. gossypii was cloned and characterized, and its relative expression level differed among instars. The expression of CYP380C12 increased by 48.5% compared with the Water group under imidacloprid (IMI) stress for 48 h. Molecular docking predicted the binding free energy of CYP380C12 and IMI was -7.0 kcal/mol, indicating an excellent stability at the docked active site, which was verified by molecular dynamics simulations. Subsequently, silencing efficiency of CYP380C12 in the dsCYP380C12 treatment group reached 48.9%, 47.0%, and 40.0% at 24, 48, and 72 h, and the mortality of A. gossypii treated with IMI was 1.45- and 1.38-fold that of the Water and dsGFP control groups, respectively. Moreover, the reproductive period of aphids treated with dsCYP380C12 (9.67 ± 0.88) was significantly shorter than that of those sprayed with Water (13.67 ± 1.20). Taken together, these results indicated that silencing CYP380C12 not only increases sensitivity of A. gossypii to IMI but also shortens its reproductive period. Understanding the function of CYP380C12 in A. gossypii can provide new insights for developing innovative strategies to control A. gossypii.
棉蚜是一种严重的害虫，会造成重大经济损失，并且由于对新烟碱类杀虫剂的抗性迅速发展而难以控制。昆虫细胞色素P450在杀虫剂解毒中起重要作用。在本研究中，我们旨在了解棉蚜中CYP380C12的功能。克隆并鉴定了棉蚜的CYP380C12基因，其相对表达水平在不同龄期有所不同。在吡虫啉（IMI）胁迫48小时后，与水对照组相比，CYP380C12的表达增加了48.5%。分子对接预测CYP380C12与IMI的结合自由能为-7.0 kcal/mol，表明在对接活性位点具有优异的稳定性，这通过分子动力学模拟得到了验证。随后，dsCYP380C12处理组中CYP380C12在24、48和72小时的沉默效率分别达到48.9%、47.0%和40.0%，用IMI处理的棉蚜死亡率分别是水对照组和dsGFP对照组的1.45倍和1.38倍。此外，用dsCYP380C12处理的蚜虫的繁殖期（9.67±0.88）明显短于喷水处理的蚜虫（13.67±1.20）。综上所述，这些结果表明沉默CYP380C12不仅增加了棉蚜对IMI的敏感性，还缩短了其繁殖期。了解CYP380C12在棉蚜中的功能可为制定控制棉蚜的创新策略提供新的见解。
