Xie Keming, Liu Chang, Liu Guangfeng, Zhu Peng, Yang Zheng, Yuan Lihong, Jiang Jingzhe
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510300, China.
School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China.
Arch Virol. 2025 Apr 25;170(6):113. doi: 10.1007/s00705-025-06291-9.
Bacteriophages that infect specific bacteria and cause their lysis are potentially useful for preventing and treating bacterial infections and controlling microbes in the environment. Citrobacter freundii is an opportunistic pathogen that is commonly present in the environment, food, animals, and human intestines. It also causes aquatic animal diseases in aquaculture. In this study, using Citrobacter freundii as the target host, a novel lytic phage, vB_CfrS_K1M (K1M), was isolated. Morphologically, K1M is a long-tailed phage with a head diameter of 68 ± 2 nm and a tail length of 130 ± 2 nm. The phage encodes a polysaccharide depolymerase and demonstrates stable biological activity. The genome of K1M is 47,995 bp in length, with 45.12% G + C content and 97 putative open reading frames (ORFs), only 36 of which encode proteins. The results of phylogenetic analysis based on TerL sequences suggest that K1M represents a new viral genus, for which we propose the name "Fredivirus".
感染特定细菌并导致其裂解的噬菌体在预防和治疗细菌感染以及控制环境中的微生物方面具有潜在用途。弗氏柠檬酸杆菌是一种机会致病菌，普遍存在于环境、食物、动物和人类肠道中。它还会在水产养殖中引发水生动物疾病。在本研究中，以弗氏柠檬酸杆菌作为靶标宿主，分离出了一种新型裂解性噬菌体vB_CfrS_K1M（K1M）。从形态学上看，K1M是一种长尾噬菌体，头部直径为68±2 nm，尾部长度为130±2 nm。该噬菌体编码一种多糖解聚酶，并表现出稳定的生物学活性。K1M的基因组长度为47,995 bp，G+C含量为45.12%，有97个推定的开放阅读框（ORF），其中只有36个编码蛋白质。基于TerL序列的系统发育分析结果表明，K1M代表一个新的病毒属，我们为此提议将其命名为“弗氏病毒属”。
