Xu Jiyi, Ge Ziyu, Wang Han, Zhang Chenhui, Xu Jinjie, Li Ying, Yang Xiangyun, Zhang Ling, Li Zhanjiang, Liu Zhe, Wang Gang, Du Jing
The National Clinical Research Center for Mental Disorders & Beijing Key Laboratory of Mental Disorders, Beijing Anding Hospital & Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing, 100088, China.
Bloomage Biotechnology Co., Ltd. BITC Build D, No. 6, Jianguomenwai Street, Chaoyang District, Beijing, 100022, China.
NPJ Sci Food. 2025 Apr 24;9(1):60. doi: 10.1038/s41538-025-00423-w.
Anxiety disorders are among the most prevalent mental health conditions, often linked with neuroinflammation and imbalances in neurotransmitter systems. This study examined the anxiolytic effects of oral GABA in chronic restraint stress (CRS) mice. Mice were divided into control, CRS, and two GABA-treated groups (10 mg/kg, 20 mg/kg). After 14 days of administration, anxiety-like behaviors were assessed using elevated-plus maze and open-field tests. GABA levels in the prefrontal cortex were quantified via ELISA, while anti-inflammatory cytokines were measured using an antibody array. Proteomic analysis of the hippocampus identified differentially expressed proteins, validated through Parallel Reaction Monitoring and immunoblotting. Results showed that GABA significantly alleviated anxiety-like behaviors, increased GABA levels in the prefrontal cortex, and elevated anti-inflammatory factors IL-10 and TGF-β1. Proteomic analysis and validation revealed GABA reversed complement dysregulation (C3, C4b, Cfh, Cfi). These findings suggest GABA alleviates anxiety by modulating immune homeostasis and complement activation, highlighting its therapeutic potential.
焦虑症是最常见的心理健康状况之一，通常与神经炎症和神经递质系统失衡有关。本研究考察了口服γ-氨基丁酸（GABA）对慢性束缚应激（CRS）小鼠的抗焦虑作用。将小鼠分为对照组、CRS组和两个GABA治疗组（10毫克/千克、20毫克/千克）。给药14天后，使用高架十字迷宫和旷场试验评估焦虑样行为。通过酶联免疫吸附测定法（ELISA）对前额叶皮质中的GABA水平进行定量，同时使用抗体阵列测量抗炎细胞因子。对海马体进行蛋白质组学分析，鉴定出差异表达的蛋白质，并通过平行反应监测和免疫印迹进行验证。结果表明，GABA显著减轻焦虑样行为，提高前额叶皮质中的GABA水平，并升高抗炎因子白细胞介素-10（IL-10）和转化生长因子-β1（TGF-β1）。蛋白质组学分析和验证显示，GABA可逆转补体失调（C3、C4b、Cfh、Cfi）。这些发现表明，GABA通过调节免疫稳态和补体激活来减轻焦虑，凸显了其治疗潜力。
