Erel Cemal Tamer, Neccar Duygu, Ozcivit Erkan Ipek Betul, Gokmen Inan Neslihan, Guzel Meydanli Elif
Department of Obstetrics and Gynecology, Cerrahpasa Faculty of Medicine, Istanbul University-Cerrahpasa, Istanbul, Türkiye.
Department of Histology and Embryology, Cerrahpasa Faculty of Medicine, Istanbul University-Cerrahpasa, Istanbul, Türkiye.
Lasers Surg Med. 2025 Jul;57(5):415-425. doi: 10.1002/lsm.70020. Epub 2025 Apr 24.
To investigate the thermo-chemical effects of nonablative Er:YAG laser on human endometrial stromal cells (hESCs).
In this laboratory based study, for the hESCs cultures, endometrial tissue biopsy samples were obtained from three fertile women in the late proliferative phase. In this laboratory-based study, seven experimental groups were created: Estradiol (E) group, E + Sham (S) group, E + P group, E + Laser (L) group, E + P + L group, hESC group and hESC+L group. Cell cultures reaching confluence on the matrigel-coated petri dishes in all groups were incubated for 12, 24, 48, and 72 h after laser irradiation, respectively. Nonablative Er:YAG laser irradiation was performed to the hESCs culture. The main outcome of this study involves assessing the alterations in MMP-2, TNF-α, IL-6, VEGF-A, and IGFBP-1 levels within conditioned media of hESC cultures subsequent to the Er:YAG laser irradiation.
Median MMP-2 levels significantly differed between groups at the 12th, 24th, 48th, and 72nd hour (p = 0.012, p = 0.045, p = 0.021, and p = 0.032, respectively). All media of hESCs irradiated with Er-YAG laser had higher median MMP-2 values. At the 48th hour, the groups showed significant differences in terms of median TNF-α levels (p = 0.029), which were lower in the groups that received Er-YAG laser. At the 12th hour, median IL-6 levels differed significantly between the groups (p = 0.011), being higher in the groups that received Er-YAG laser. At the 72nd hour, lower median VEGF-A values were observed for the groups that received Er-YAG laser (p = 0.021). A statistically significant difference existed in terms of median IGFBP-1 levels between the groups at the 24th hour, with higher levels in the groups which received Er-YAG laser (p = 0.041).
Our findings demonstrated that the non-ablative Er-YAG laser induces tissue remodeling, modulates immune responses by favoring Th2 cell activity and suppresses Th1 cell activity in hESCs. Additionally, it exerts favorable impact on decidualization.
研究非剥脱性铒激光对人子宫内膜基质细胞（hESCs）的热化学效应。
在这项基于实验室的研究中，为培养hESCs，从三名处于增殖晚期的育龄妇女获取子宫内膜组织活检样本。在这项基于实验室的研究中，创建了七个实验组：雌二醇（E）组、E + 假手术（S）组、E + 孕酮（P）组、E + 激光（L）组、E + P + L组、hESC组和hESC + L组。所有组在基质胶包被的培养皿中达到汇合的细胞培养物在激光照射后分别孵育12、24、48和72小时。对hESCs培养物进行非剥脱性铒激光照射。本研究的主要结果包括评估铒激光照射后hESC培养物条件培养基中MMP - 2、TNF - α、IL - 6、VEGF - A和IGFBP - 1水平的变化。
在第12、24、48和72小时，各组间MMP - 2水平中位数有显著差异（分别为p = 0.012、p = 0.045、p = 0.021和p = 0.032）。所有接受铒激光照射的hESCs培养基中MMP - 2中位数较高。在第48小时，各组间TNF - α水平中位数有显著差异（p = 0.029），接受铒激光照射的组较低。在第12小时，各组间IL - 6水平中位数有显著差异（p = 0.011），接受铒激光照射的组较高。在第72小时，接受铒激光照射的组VEGF - A中位数较低（p = 0.021）。在第24小时，各组间IGFBP - 1水平中位数有统计学显著差异，接受铒激光照射的组水平较高（p = 0.041）。
我们的研究结果表明，非剥脱性铒激光可诱导组织重塑，通过促进Th2细胞活性调节免疫反应并抑制hESCs中的Th1细胞活性。此外，它对蜕膜化有有利影响。
