α-促黑素对体外培养的黑色素瘤细胞酪氨酸酶活性及黑色素积累速率的影响。
Effect of alpha-MSH on the tyrosinase activity and the rate of melanin accumulation of melanoma cells in vitro.
作者信息
Lee T H, Lee M S
出版信息
Acta Endocrinol (Copenh). 1977 Mar;84(3):663-72. doi: 10.1530/acta.0.0840663.
A stimulation of the tyrosinase activity was observed when melanoma cells isolated from transplantable mouse melanomas were incubated at 37 degrees C for 6 h in the presence of 1-10 X 10(-6) M alpha-MSH. All strains of mouse melanomas studied (B-16, Cloudman S-91 and Harding-Passey), exhibited similar responses. It was also observed that the intact cellular structure of melanoma cells was not required for the ability to respond to alpha-MSH. The stimulation of the enzymic activity was accompanied by an increase of the rate of incorporation of radioactivity into melanin from L-[U-14C]tyrosine, indicating an enhanced melanogenesis of tumour cells under in vitro conditions.
当从可移植小鼠黑色素瘤中分离出的黑色素瘤细胞在37℃下于1-10×10(-6)M α-促黑素（α-MSH）存在的条件下孵育6小时时，观察到酪氨酸酶活性受到刺激。所研究的所有小鼠黑色素瘤菌株（B-16、Cloudman S-91和Harding-Passey）均表现出类似的反应。还观察到，黑色素瘤细胞对α-MSH作出反应的能力并不需要完整的细胞结构。酶活性的刺激伴随着放射性从L-[U-14C]酪氨酸掺入黑色素的速率增加，这表明在体外条件下肿瘤细胞的黑色素生成增强。
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