一种新型垂体蛋白（7B2）在大鼠脑中的区域分布。

Regional distribution of a novel pituitary protein (7B2) in the rat brain.

作者信息

Iguchi H, Chan J S, Dennis M, Seidah N G, Chrétien M

出版信息

Brain Res. 1985 Jul 8;338(1):91-6. doi: 10.1016/0006-8993(85)90251-3.

Abstract

The regional distribution of a novel pituitary protein (7B2) in the rat brain was studied using a specific and sensitive radioimmunoassay. Immunoreactive (IR)-7B2 was distributed throughout the brain, with the highest concentrations in the pituitary, hypothalamus and basal ganglia. Immunoreactive 7B2 from the brain and other tissues had an apparent molecular weight of around 20,000 as estimated by SDS-polyacrylamide gel electrophoresis as observed with other tissues. In homozygous Brattleboro rats which do not synthesize vasopressin or its associated neurophysin, IR-7B2 levels in the brain and pituitary gland were shown to be similar to those of control animals. Furthermore, the molecular weight of 7B2 in the brain and pituitary gland of homozygous Brattleboro rats was similar to that of control animals.

摘要

利用一种特异且灵敏的放射免疫分析法，研究了一种新型垂体蛋白（7B2）在大鼠脑中的区域分布。免疫反应性（IR）-7B2分布于整个大脑，在垂体、下丘脑和基底神经节中的浓度最高。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳估计，大脑和其他组织中的免疫反应性7B2的表观分子量约为20,000，这与在其他组织中观察到的情况一致。在不合成加压素或其相关神经垂体素的纯合布拉特洛维大鼠中，大脑和垂体中的IR-7B2水平与对照动物相似。此外，纯合布拉特洛维大鼠大脑和垂体中7B2的分子量与对照动物相似。