Murphy S, Rudge J
Brain Res. 1985 Jul;353(1):73-81. doi: 10.1016/0165-3806(85)90024-0.
Twenty-day-old reeler and normal mice were either injected intraventricularly with radiolabelled fucose before subcellular fractionation of the cerebral cortex followed by SDS-PAGE, or gels of such fractions were overlaid with [125I]concanavalin A (ConA). While there were no differences in polypeptide profiles of normal and reeler subcellular fractions there were marked differences in the abundance of particular ConA-binding glycoproteins and in fucose incorporation into particular glycoproteins, especially in the synaptic plasma membrane (SPM) and 100,000 g soluble fractions. In addition there was a significantly lower binding (greater than 50%) of quinuclidinyl benzilate to reeler microsomal and SPM fractions as compared with normal. The differences in glycoprotein expression may be pertinent to anatomical observations of abnormal interactions between neurons and glial fibres during development of the reeler cerebrum.
在对20日龄的震颤小鼠和正常小鼠的大脑皮层进行亚细胞分级分离并随后进行SDS-PAGE之前，向其脑室内注射放射性标记的岩藻糖，或者将这些分级分离物的凝胶用[125I]伴刀豆球蛋白A（ConA）覆盖。虽然正常和震颤小鼠亚细胞分级分离物的多肽谱没有差异，但特定ConA结合糖蛋白的丰度以及岩藻糖掺入特定糖蛋白的情况存在显著差异，尤其是在突触质膜（SPM）和100,000 g可溶级分中。此外，与正常小鼠相比，震颤小鼠微粒体和SPM级分对奎宁环基苯甲酸酯的结合显著降低（超过50%）。糖蛋白表达的差异可能与震颤小鼠大脑发育过程中神经元与神经胶质纤维之间异常相互作用的解剖学观察结果相关。
