Paleekui Puchcharin, Rattanamato Benjamard, Kanha Nattapong, Rakariyatham Kanyasiri, Klangpetch Wannaporn, Osiriphun Sukhuntha, Laokuldilok Thunnop
Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand.
Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand.
Gels. 2025 Apr 20;11(4):303. doi: 10.3390/gels11040303.
Dysphagia, a condition characterized by difficulty swallowing, is commonly found in the elderly, increasing the risk of choking and aspiration. This study aimed to develop a texturally modified soy protein gel that meets the Universal Design Food (UDF) standard, specifically for elderly individuals with dysphagia. To create soft-textured foods, the gel's texture was modified using transglutaminase at varying concentrations (0.5%, 1.0%, and 2.0%, /) in combination with agar. The soy protein gel prepared with 0.5% transglutaminase exhibited the lowest hardness value (2.2 × 10 N/m) and was classified as Level 4 under the UDF standard, indicating that it requires no chewing and is easy to swallow, making it suitable for individuals with severe dysphagia. In contrast, the gel formulated with 2.0% transglutaminase and 0.5% agar had the highest hardness value (3.29 × 10 N/m) and was classified as Level 2, meaning it can be easily mashed with the gums, making it appropriate for individuals with moderate dysphagia. Structural analyses revealed that modifying with transglutaminase altered the protein's secondary structure by reducing the content of α-helixes and β-sheets while increasing β-turns, potentially enhancing gel network flexibility. These findings suggest that the combined use of transglutaminase and agar effectively modifies soy protein gel texture to meet the dietary needs of elderly individuals with dysphagia. This approach shows promise for the food industry by providing safer and more diverse food options for aging populations facing dysphagia.
吞咽困难是一种以吞咽困难为特征的病症,常见于老年人,会增加窒息和误吸的风险。本研究旨在开发一种符合通用设计食品(UDF)标准的质地改良大豆蛋白凝胶,专门针对患有吞咽困难的老年人。为了制作质地柔软的食物,使用不同浓度(0.5%、1.0%和2.0%,/)的转谷氨酰胺酶与琼脂结合来改良凝胶的质地。用0.5%转谷氨酰胺酶制备的大豆蛋白凝胶表现出最低的硬度值(2.2×10 N/m),根据UDF标准被归类为4级,这表明它无需咀嚼且易于吞咽,适合重度吞咽困难的个体。相比之下,用2.0%转谷氨酰胺酶和0.5%琼脂配制的凝胶具有最高的硬度值(3.29×10 N/m),被归类为2级,这意味着它可以很容易地用牙龈捣碎,适合中度吞咽困难的个体。结构分析表明,用转谷氨酰胺酶进行改性会改变蛋白质的二级结构,通过减少α-螺旋和β-折叠的含量,同时增加β-转角,可能增强凝胶网络的柔韧性。这些发现表明,转谷氨酰胺酶和琼脂的联合使用有效地改良了大豆蛋白凝胶的质地,以满足患有吞咽困难的老年人的饮食需求。这种方法为食品工业带来了希望,为面临吞咽困难的老年人群提供了更安全、更多样化的食物选择。