神经氨酸酶处理的兔红细胞和网织红细胞的体内行为。

In vivo behaviour of neuraminidase-treated rabbit erythrocytes and reticulocytes.

作者信息

Balduini C L, Ricevuti G, Sosso M C, Ascari E, Brovelli A, Balduini C

出版信息

Acta Haematol. 1977;57(3):178-87. doi: 10.1159/000207879.

DOI:10.1159/000207879

Abstract

51Cr rabbit erythrocytes were treated with different amount of neuraminidase and reinjected into the animal. The survival curves after the removal of more than 50% membrane sialic acid show a characteristic behaviour: after a rapid decrease, blood radioactivity increases again reaching a maximum level 50-80 h after reinjection, then tends to decrease with a slope similar to that of control curves. Liver radioactivity determined before the rise of blood radioactivity is evidently higher than the value determined after radioactivity elevation. Similar results were obtained with phenylhydrazine-induced young erythrocytes.

摘要

用不同量的神经氨酸酶处理51Cr标记的兔红细胞，然后再将其重新注入动物体内。去除超过50%的膜唾液酸后的存活曲线呈现出一种特征性行为：在快速下降之后，血液放射性再次增加，在重新注入后50 - 80小时达到最高水平，然后趋于下降，其斜率与对照曲线相似。在血液放射性升高之前测定的肝脏放射性明显高于放射性升高后测定的值。用苯肼诱导的幼红细胞也得到了类似的结果。