Bloch Sylwia, Sinden Richard R, Wien Frank, Węgrzyn Grzegorz, Arluison Véronique
Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland.
Department of Chemistry, Biology and Health Sciences, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA.
Membranes (Basel). 2025 Apr 1;15(4):103. doi: 10.3390/membranes15040103.
DNA metabolism consists of crucial processes occurring in all living cells. These processes include various transactions, such as DNA replication, genetic recombination, transposition, mutagenesis, and DNA repair. While it was initially assumed that these processes might occur in the cytoplasm of prokaryotic cells, subsequent reports indicated the importance of the cell membrane in various DNA transactions. Furthermore, newly identified factors play significant roles in regulating DNA-related cellular processes. One such factor is the Hfq protein, originally discovered as an RNA chaperone but later shown to be involved in several molecular mechanisms. These include DNA transactions and interaction with the cell membrane. Recent studies have suggested that Hfq plays a role in the regulation of DNA replication, mutagenesis, and recombination. In this narrative review, we will focus on the importance of membranes in DNA transactions and discuss the potential role of Hfq-mediated regulation of these processes in , where the protein is the best characterized. Special attention is given to the affinity of this small protein for both DNA and membranes, which might help explain some of the findings from recent experiments.
DNA代谢由所有活细胞中发生的关键过程组成。这些过程包括各种活动，如DNA复制、基因重组、转座、诱变和DNA修复。虽然最初认为这些过程可能发生在原核细胞的细胞质中，但随后的报告表明细胞膜在各种DNA活动中的重要性。此外，新发现的因子在调节与DNA相关的细胞过程中发挥着重要作用。其中一个因子是Hfq蛋白，最初被发现是一种RNA伴侣，但后来发现它参与了多种分子机制。这些机制包括DNA活动以及与细胞膜的相互作用。最近的研究表明，Hfq在DNA复制、诱变和重组的调节中发挥作用。在这篇叙述性综述中，我们将重点关注膜在DNA活动中的重要性，并讨论Hfq介导的这些过程调节在[具体物种]中的潜在作用，在该物种中该蛋白的特征最为明确。特别关注这种小蛋白对DNA和膜的亲和力，这可能有助于解释最近实验中的一些发现。
