一项为期24周的有监督的同时进行的运动干预对久坐不动的年轻成年人粪便微生物群多样性和组成的影响：ACTIBATE随机对照试验。
Effect of a 24-week supervised concurrent exercise intervention on fecal microbiota diversity and composition in young sedentary adults: The ACTIBATE randomized controlled trial.
作者信息
Martinez-Tellez Borja, Xu Huiwen, Ortiz-Alvarez Lourdes, Rodríguez-García Carmen, Schönke Milena, Jurado-Fasoli Lucas, Osuna-Prieto Francisco J, Alcantara Juan M A, Acosta Francisco M, Amaro-Gahete Francisco J, Folkerts Gert, Vilchez-Vargas Ramiro, Link Alexander, Plaza-Diaz Julio, Gil Angel, Labayen Idoia, Fernandez-Veledo Sonia, Rensen Patrick C N, Ruiz Jonatan R
机构信息
Department of Physical Education and Sports, Faculty of Sports Science, Sport and Health University Research Institute (iMUDS), University of Granada, 18071, Granada, Spain; Department of Medicine, Division of Endocrinology, and Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, 2333 ZA, Leiden, the Netherlands; CIBEROBN, Biomedical Research Networking Center for Physiopathology of Obesity and Nutrition, Carlos III Health Institute, 18100, Granada, Spain; Department of Nursing, Physiotherapy and Medicine and SPORT Research Group, CIBIS Research Center, University of Almería, 04120, Almería, Spain.
Department of Physical Education and Sports, Faculty of Sports Science, Sport and Health University Research Institute (iMUDS), University of Granada, 18071, Granada, Spain; Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, 1807, Granada, Spain.
出版信息
Clin Nutr. 2025 Jun;49:128-137. doi: 10.1016/j.clnu.2025.04.008. Epub 2025 Apr 15.
BACKGROUND
Numerous physiological responses to exercise are observed in humans, yet the effects of long-term exercise and varying intensities on the diversity and composition of human fecal microbiota remain unclear. We investigated the effect of a 24-week supervised concurrent exercise intervention, at moderate and vigorous intensities, on fecal microbiota diversity and composition in young adults.
METHODS
This ancillary study was based on data from the ACTIBATE randomized controlled trial (ClinicalTrials.gov ID: NCT02365129), and included adults (aged 18-25 years, 70 % female) that were randomized to (i) a control group (CON: no exercise, n = 20), (ii) a moderate-intensity exercise group (MOD-EX, n = 21), and (iii) a vigorous-intensity exercise group (VIG-EX, n = 20). Fecal samples were collected before and after the 24-week exercise intervention, and the diversity and composition of the fecal microbiota were analyzed by 16S rRNA sequencing. Inferential functional profiling of the fecal microbiota was performed and correlations between microbial changes and cardiometabolic outcomes were assessed.
RESULTS
Exercise did not modify beta or alpha diversities regardless of the intensity (all P ≥ 0.062). The relative abundance of the Erysipelotrichaceae family (Bacillota phylum) (-0.3 ± 1.2 %; P = 0.031) was however reduced in the VIG-EX group. Coprococcus was the only genus showed a significant difference between MOD-EX and VIG-EX after the intervention, with its relative abundance increasing in MOD-EX (+0.4 ± 0.6 %; P = 0.005). None of these changes were related to the exercise-induced cardiometabolic benefits (all P ≥ 0.05).
CONCLUSIONS
In young adults, a 24-week supervised concurrent exercise program, at moderate and vigorous intensities, resulted in minor changes in fecal microbiota composition, while neither alpha nor beta diversities were affected.
CLINICAL TRIAL REGISTRATION
ClinicalTrials.gov ID: NCT02365129.
背景
在人类中观察到了许多对运动的生理反应，但长期运动以及不同强度的运动对人类粪便微生物群的多样性和组成的影响仍不清楚。我们研究了一项为期24周的有监督的同时进行的运动干预，包括中等强度和高强度运动，对年轻成年人粪便微生物群多样性和组成的影响。
方法
这项辅助研究基于ACTIBATE随机对照试验（ClinicalTrials.gov标识符：NCT02365129）的数据，纳入了成年人（年龄18 - 25岁，70%为女性），他们被随机分为：（i）对照组（CON：不运动，n = 20），（ii）中等强度运动组（MOD - EX，n = 21），以及（iii）高强度运动组（VIG - EX，n = 20）。在24周运动干预前后收集粪便样本，并通过16S rRNA测序分析粪便微生物群的多样性和组成。对粪便微生物群进行推断性功能分析，并评估微生物变化与心脏代谢结果之间的相关性。
结果
无论运动强度如何，运动均未改变β或α多样性（所有P≥0.062）。然而，高强度运动组中丹毒丝菌科（厚壁菌门）的相对丰度降低了（-0.3±1.2%；P = 0.031）。干预后，粪球菌是中等强度运动组和高强度运动组之间唯一显示出显著差异的属，其相对丰度在中等强度运动组中增加（+0.4±0.6%；P = 0.005）。这些变化均与运动诱导的心脏代谢益处无关（所有P≥0.05）。
结论
在年轻成年人中，一项为期24周的有监督的同时进行的运动计划，包括中等强度和高强度运动，导致粪便微生物群组成有微小变化，而α和β多样性均未受到影响。
临床试验注册
ClinicalTrials.gov标识符：NCT02365129。
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