用亚硝酸降解牛肺肝素得到的二硫酸化二糖的核磁共振研究。
N.m.r. studies of the disulphated disaccharide obtained by degradation of bovine lung heparin with nitrous acid.
作者信息
Huckerby T N, Sanderson P N, Nieduszynski I A
出版信息
Carbohydr Res. 1985 May 15;138(2):199-206. doi: 10.1016/0008-6215(85)85103-x.
The disulphated disaccharide IdoA(2SO3)-anManOH(6SO3) was prepared from bovine lung heparin by treatment with nitrous acid followed by borohydride reduction. The 1H- (400 MHz) and 13C-n.m.r. (100 MHz) spectra of this disaccharide derivative have been assigned completely using homonuclear spin-decoupling experiments, 13C-1H correlations, and a COSY-45 two-dimensional homonuclear correlation experiment. The 3JH,H values show that the IdoA(2SO3) residue exists in a single conformation throughout the temperature range 20-90 degrees.
通过用亚硝酸处理牛肺肝素，随后进行硼氢化物还原，制备出了二硫酸化二糖艾杜糖醛酸（2 - 磺酸基）-α-甘露糖醇（6 - 磺酸基）。利用同核自旋去耦实验、¹³C - ¹H 相关以及COSY - 45二维同核相关实验，已完全归属了该二糖衍生物的¹H - （400 MHz）和¹³C - NMR（核磁共振，100 MHz）谱。³JH,H值表明，在20 - 90摄氏度的整个温度范围内，艾杜糖醛酸（2 - 磺酸基）残基以单一构象存在。
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