Yang Yurong, Ma Yiheng, Quan Kai, Guo Bingyan, Jiang Yibao
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, China.
College of Animal Science, Henan University of Animal Husbandry and Economy, Zhengzhou 450000, China.
Animals (Basel). 2025 Apr 8;15(8):1074. doi: 10.3390/ani15081074.
Ovine toxoplasmosis has a significant negative impact on sheep farming, and abortion is the main clinical manifestation. The objective of the present study was to survey ovine toxoplasmosis and assess its potential harm in sheep. Sheep serum or heart fluid was collected from 1035 sheep, along with the tissue or blood samples collected from 164 of them. We investigated ovine toxoplasmosis by a modified agglutination test (MAT) ( = 1035 sheep) and by a polymerase chain reaction (PCR) ( = 164 sheep) in China. The results showed that 75 sheep were seropositive (titer ≥ 1:100), with a prevalence of 7.2%, as assessed by MAT. The molecular prevalence of in sheep was 16.4% (27/164), as assessed by PCR. From these 164 tissue or blood samples, 22 tissues and 2 blood samples were selected in order to isolate by mouse bioassay. A viable strain (TgSheepCHn15, ToxoDB #10) was isolated from sheep tissues in the veterinary clinic. This strain was avirulent for Swiss mice, and the survival time of mice was 97 ± 31 days. This is the first instance of the isolation of a Type I strain from sheep with avirulence in mice. The prevalence of has been decreasing in sheep from China. However, remains present in sheep herds and should not be ignored.
绵羊弓形虫病对养羊业有重大负面影响，流产是主要临床表现。本研究的目的是调查绵羊弓形虫病并评估其对绵羊的潜在危害。从1035只绵羊中采集血清或心脏液，同时从其中164只绵羊采集组织或血液样本。在中国，我们采用改良凝集试验（MAT）（n = 1035只绵羊）和聚合酶链反应（PCR）（n = 164只绵羊）对绵羊弓形虫病进行调查。结果显示，通过MAT评估，75只绵羊血清呈阳性（滴度≥1:100），患病率为7.2%。通过PCR评估，绵羊弓形虫的分子患病率为16.4%（27/164）。从这164份组织或血液样本中，选取22份组织和2份血液样本，通过小鼠生物测定法分离弓形虫。在兽医诊所从绵羊组织中分离出一株活的弓形虫菌株（TgSheepCHn15，ToxoDB #10）。该菌株对瑞士小鼠无毒力，小鼠存活时间为97±31天。这是首次从绵羊中分离出对小鼠无毒力的I型菌株。中国绵羊中弓形虫的患病率一直在下降。然而，弓形虫仍存在于羊群中，不应被忽视。
