一种使用逆转录聚合酶链置换反应（RT-PSR）和羟基萘酚蓝指示剂检测活菌的视觉检测方法的开发。
Development of a Visual Assay for Detection of Viable Using RT-PSR and Hydroxynaphthol Blue Indicator.
作者信息
Wang Peng, Chen Qiming, Wang Yikai, Sun Xueting, Liu Zhanmin
机构信息
School of Life Sciences, Shanghai University, No. 99 Shangda Road, Shanghai 200444, China.
Nantong Customs of the People's Republic of China, No. 102 Chongchuan Road, Nantong 226006, China.
出版信息
Biology (Basel). 2025 Apr 7;14(4):383. doi: 10.3390/biology14040383.
is a foodborne pathogen in powdered infant formula, which poses a significant risk to susceptible populations such as infants and the elderly. This study aims to develop a visual detection method for viable using the reverse transcription-polymerase spiral reaction and hydroxynaphthol blue indicator. Under the optimized conditions, the detection process could be completed within 55 min with low equipment dependence. It was evaluated to have high specificity and sensitivity with the detection limit low to 1.2 × 10 CFU/mL. The assay also showed 100% accuracy in artificially contaminated samples.
是婴儿配方奶粉中的一种食源性病原体，对婴儿和老年人等易感人群构成重大风险。本研究旨在利用逆转录-聚合酶螺旋反应和羟基萘酚蓝指示剂开发一种用于活菌的视觉检测方法。在优化条件下，检测过程可在55分钟内完成，对设备依赖性低。经评估，该方法具有高特异性和高灵敏度，检测限低至1.2×10 CFU/mL。该检测方法在人工污染样本中也显示出100%的准确率。 （原文中“1.2 × 10 CFU/mL”表述有误，推测可能是“1.2 × 10⁴ CFU/mL”之类，可根据实际情况修正）
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