Zhang Qun, Xu Xiuyue, Yang Ling
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
College of Forestry, Beijing Forestry University, Beijing 100091, China.
Biology (Basel). 2025 Apr 12;14(4):411. doi: 10.3390/biology14040411.
(1) Background: (WUSCHEL-related homologous box) is a plant-specific transcription factor involved in plant development and stress response. It has been reported to be involved in processes such as growth and development, stem cell division and differentiation, and organ development; (2) Methods: In this study, bioinformatics was used to identify and analyze the gene family of Korean pine. The gene characteristics were identified and analyzed through yeast transcriptional activation assays as well as subcellular localization experiments; (3) Results: A total of 21 members of the gene family of Korean pine were identified in this study. The phylogenetic tree divides the genes into three sub-branches. 21 genes are unevenly distributed on 7 of the 12 chromosomes. was expressed in all tissues. , had higher expression in the embryonic callus, non-embryonic callus, somatic embryo, and zygotic embryo. , and were located in the nucleus and in the cell membrane. The and proteins exhibited transcriptional self-activation activity, while did not; (4) Conclusions: In this study, the members of the WOX transcription factor family in Korean pine were identified and systematically analyzed, laying a foundation for their subsequent functional research.
(1) 背景：WUSCHEL相关同源框（WUSCHEL-related homologous box，WOX）是一种参与植物发育和胁迫响应的植物特异性转录因子。据报道，它参与生长发育、干细胞分裂与分化以及器官发育等过程；(2) 方法：在本研究中，利用生物信息学方法对红松WOX基因家族进行鉴定与分析。通过酵母转录激活实验以及亚细胞定位实验对基因特性进行鉴定与分析；(3) 结果：本研究共鉴定出红松WOX基因家族的21个成员。系统发育树将WOX基因分为三个亚分支。21个WOX基因不均匀地分布在12条染色体中的7条上。WOX在所有组织中均有表达。PwWOX11、PwWOX12在胚性愈伤组织、非胚性愈伤组织、体细胞胚和合子胚中具有较高表达。PwWOX3、PwWOX9和PwWOX13定位于细胞核和细胞膜。PwWOX9和PwWOX11蛋白表现出转录自激活活性，而PwWOX13则没有；(4) 结论：本研究对红松WOX转录因子家族成员进行了鉴定和系统分析，为其后续功能研究奠定了基础。
