Purification, Structural Analysis and Bioactivity of Polysaccharide.

is a medicinal and edible plant widely distributed in Asia. It has antipyretic, analgesic, and anti-inflammatory properties. In this study, a novel polysaccharide (PMPS-A) was obtained through purification, and its biological activity was investigated. Structural analysis revealed that PMPS-A was composed of fructose and glucose, with a molecular weight of 12168 Da. The main chain structure was →1)-β-D-Fru-(2→, →4)-α-D-Glc-(1→, →4)-α-D-Glc-(1→, and →4)-α-D-Glc-(1→. The branched chain of α-D-Glc-(1→3)-α-D-Glc-(1→ and α-D-Glc-(1→ connected to the -3 and -6 positions of residue →3,4)-α-D-Glc-(1→ and →4,6)-α-D-Glcp-(1→, respectively. In vitro, PMPS-A had a favorable scavenging ability of the hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and downregulated the expression of interleukin-6 and nitric oxide in lipopolysaccharide-induced RAW264.7 macrophages. In addition, the Caenorhabditis model assay demonstrated that PMPS-A decreased the buildup of lipofuscin and reactive oxygen species. Overall, these results enhance our knowledge of the chemical composition and bioactivity of a polysaccharide and point to the potential use of PMPS-A for antioxidant and anti-aging qualities, providing a theoretical basis for the medicinal and edible application of polysaccharide.