Dong Yihan, Zhang Luyao, Qiu Di, Yao Renxin, Jia Haitao, Wang Haiyang, Zhou Luyao, Zhang Jiantao, Zhang Na
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and Comparative Medicine, Harbin 150030, China.
Foods. 2025 Apr 19;14(8):1416. doi: 10.3390/foods14081416.
Cyclophosphamide (CTX) is a widely used anticancer drug in clinical practice; however, its administration can lead to gastrointestinal damage and immune suppression. () has been shown to regulate immune cell activity and protect the gastrointestinal system, showing potential application as a functional food. The objective of this study was to investigate the effects of ZNL-13 on CTX-induced intestinal mucosal injury and gut microbiota in mice. The results demonstrated that ZNL-13 significantly alleviated weight loss and intestinal pathological damage. Moreover, in CTX-induced intestinal injury mice, ZNL-13 enhanced the release of immune factors, suppressed cell apoptosis, and protected the intestinal mucosal barrier. Additionally, it activated the TLR4/NF-κB pathway, thereby promoting immune cell activity. Furthermore, ZNL-13 contributed to the restoration of gut microbial homeostasis by increasing the relative abundance of short-chain fatty acid-producing bacteria. Taken together, this investigation highlights the potential of ZNL-13 in protecting the intestinal barrier and enhancing immune function while laying the groundwork for its development as a novel probiotic and functional food.
环磷酰胺（CTX）是临床实践中广泛使用的抗癌药物；然而，其给药会导致胃肠道损伤和免疫抑制。（）已被证明可调节免疫细胞活性并保护胃肠道系统，显示出作为功能性食品的潜在应用价值。本研究的目的是探讨ZNL-13对CTX诱导的小鼠肠道黏膜损伤和肠道微生物群的影响。结果表明，ZNL-13显著减轻体重减轻和肠道病理损伤。此外，在CTX诱导的肠道损伤小鼠中，ZNL-13增强免疫因子的释放，抑制细胞凋亡，并保护肠道黏膜屏障。此外，它激活TLR4/NF-κB途径，从而促进免疫细胞活性。此外，ZNL-13通过增加产生短链脂肪酸的细菌的相对丰度，有助于恢复肠道微生物稳态。综上所述，本研究突出了ZNL-13在保护肠道屏障和增强免疫功能方面的潜力，同时为其作为新型益生菌和功能性食品的开发奠定了基础。
