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频率调节的重复微振动通过BMP信号通路促进MC3T3-E1细胞的成骨细胞分化

Frequency-Regulated Repeated Micro-Vibration Promotes Osteoblast Differentiation Through BMP Signaling in MC3T3-E1 Cells.

作者信息

Matsushita Ayumu, Kudo Tada-Aki, Tominami Kanako, Hayashi Yohei, Noguchi Takuya, Tanaka Takakuni, Izumi Satoshi, Gengyo-Ando Keiko, Matsuzawa Atsushi, Hong Guang, Nakai Junichi

机构信息

Division of Oral Physiology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan.

Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan.

出版信息

Life (Basel). 2025 Apr 3;15(4):588. doi: 10.3390/life15040588.

DOI:10.3390/life15040588
PMID:40283143
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12028574/
Abstract

Physical stimulation, which is a key factor affecting the metabolism of osteoblasts and their precursor cells, plays an important role in bone remodeling; however, the role of micro-vibrations in osteoblast differentiation is unclear. In the present study, we determined the effects of frequency-regulated repeated micro-vibration (FRMV) on cell proliferation and established a method to induce osteoblast differentiation through FRMV using the mouse pre-osteoblast-like cell line MC3T3-E1, which is widely used in bone metabolism research. The results indicated that FRMV significantly influenced the proliferation of MC3T3-E1 cells in a normal growth medium. FRMV at 42.2 Hz significantly promoted proliferation, whereas FRMV at 92.1 Hz showed no effect on the proliferation rate. Moreover, FRMV at 42.2 Hz significantly increased alkaline phosphatase (ALP) enzyme activity and ALP gene expression in MC3T3-E1 cells. Treatment with LDN193189, a bone morphogenetic protein (BMP) signaling inhibitor, revealed that the FRMV-induced upregulation in ALP enzyme activity and ALP gene expression were significantly suppressed in MC3T3-E1 cells. The results suggest that the FRMV protocol developed in the present study induces osteoblast differentiation through the BMP signaling pathway. Thus, FRMV may contribute to the development of effective bone regeneration technologies.

摘要

物理刺激是影响成骨细胞及其前体细胞代谢的关键因素，在骨重塑中发挥着重要作用；然而，微振动在成骨细胞分化中的作用尚不清楚。在本研究中，我们使用广泛应用于骨代谢研究的小鼠前成骨样细胞系MC3T3-E1，确定了频率调节重复微振动（FRMV）对细胞增殖的影响，并建立了一种通过FRMV诱导成骨细胞分化的方法。结果表明，FRMV在正常生长培养基中显著影响MC3T3-E1细胞的增殖。42.2Hz的FRMV显著促进增殖，而92.1Hz的FRMV对增殖率没有影响。此外，42.2Hz的FRMV显著增加了MC3T3-E1细胞中碱性磷酸酶（ALP）的酶活性和ALP基因表达。用骨形态发生蛋白（BMP）信号抑制剂LDN193189处理后发现，MC3T3-E1细胞中FRMV诱导的ALP酶活性和ALP基因表达上调被显著抑制。结果表明，本研究中开发的FRMV方案通过BMP信号通路诱导成骨细胞分化。因此，FRMV可能有助于有效骨再生技术的发展。

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本文引用的文献

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