Eto M, Watanabe K, Ishii K
Clin Chim Acta. 1985 Jun 30;149(1):21-8. doi: 10.1016/0009-8981(85)90269-4.
A rapid flat gel isoelectric focusing method has been developed for the determination of apolipoprotein E phenotypes. Isoelectric focusing in 5% polyacrylamide flat gel with 8 mol/l urea and 2.8% pharmalyte (pH 4-6.5) was carried out at 3,000 V and 4 degrees C for 1 h under a constant power of 30 W. The separation of apolipoprotein E isoproteins was good and the isoelectric points were determined. Using this method, the apolipoprotein E phenotype frequency was examined in the Japanese population, and a higher frequency of phenotype E3/3 and a lower frequency of phenotype E3/2 were found in Japanese than those reported for the German, American or English population. In our focusing system the cut-off point of apolipoprotein E2/E3 ratio between the phenotype E3/3 and E3/2 was assumed to be approximately 0.9. These results indicate that this method may be useful for the determination of apolipoprotein E phenotypes.
已开发出一种快速平板凝胶等电聚焦法用于测定载脂蛋白E表型。在含有8 mol/l尿素和2.8% Pharmalyte（pH 4 - 6.5）的5%聚丙烯酰胺平板凝胶中进行等电聚焦，于3000 V和4℃下在30 W恒定功率下进行1小时。载脂蛋白E同工蛋白的分离效果良好并测定了等电点。使用该方法，在日本人群中检测了载脂蛋白E表型频率，发现日本人中E3/3表型频率较高，E3/2表型频率低于德国、美国或英国人群报道的频率。在我们的聚焦系统中，表型E3/3和E3/2之间载脂蛋白E2/E3比率的截断点假定约为0.9。这些结果表明该方法可能有助于载脂蛋白E表型的测定。
