Nakamura J, Yakata M
Clin Chim Acta. 1985 Jul 15;149(2-3):215-24. doi: 10.1016/0009-8981(85)90335-3.
A method using high performance liquid chromatography for the quantitative determination of cortisol and 6 beta-hydroxycortisol in urine is described. Urine extracts were fractionated by high performance liquid chromatography. The peak of 6 beta-hydroxycortisol was identified on the basis of retention time (24.4 min) and its area was measured. The peak of cortisol could not be measured simultaneously with the same column because of interference by other absorbing materials. The cortisol and dexamethasone peaks were collected (Rt values 9.6 and 8.1 min, respectively), rechromatographed on another column system, and the concentration was determined. Precision and accuracy of the present method were within the range commonly achieved by other methods established for both steroids. Clinical utility of the present method was evaluated by measuring urinary cortisol and 6 beta-hydroxycortisol in normal subjects, in Cushing's syndrome and disease patients, and in patients receiving cortisol therapy.
描述了一种使用高效液相色谱法定量测定尿中皮质醇和6β-羟基皮质醇的方法。尿液提取物通过高效液相色谱法进行分离。根据保留时间（24.4分钟）鉴定6β-羟基皮质醇的峰，并测量其面积。由于其他吸收物质的干扰，无法使用同一色谱柱同时测量皮质醇的峰。收集皮质醇和地塞米松的峰（保留时间分别为9.6和8.1分钟），在另一个色谱柱系统上重新进行色谱分析，并测定浓度。本方法的精密度和准确度在其他针对这两种类固醇建立的方法通常达到的范围内。通过测量正常受试者、库欣综合征和疾病患者以及接受皮质醇治疗的患者的尿皮质醇和6β-羟基皮质醇来评估本方法的临床实用性。
