Yu Ying, Shangguan Maosen, Sun Ping, Lin Xiaofeng, Li Jiqiu
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, The Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies, College of the Environment and Ecology, Xiamen University, Xiamen 361102, China.
Nansha Islands Coral Reef Ecosystem National Observation and Research Station, Guangzhou 510300, China.
Microorganisms. 2025 Mar 24;13(4):727. doi: 10.3390/microorganisms13040727.
The diurnal cycle of light significantly impacts microbes, making diurnal investigations crucial for understanding microbial communities. Zhubi Reef is known to harbor exceptionally rich biodiversity, with both zooplankton and seawater properties demonstrating diurnal patterns. However, microbial community structures and their potential diurnal dynamics remain largely unexplored. This study is the first to utilize flow cytometry and high-throughput sequencing to investigate prokaryotic and microeukaryotic communities in the Zhubi lagoon, focusing on diurnal variations under different light intensities. The picophytoplankton cell abundance and the microbial community structures both exhibit clear diurnal variations. Light is identified as the primary driver of diurnal variations in the picophytoplankton cell abundance. The diurnal variation in microbial community diversity is driven by changes in the cell abundance of two dominant picocyanobacterial groups. Our findings reveal the diurnal variation in microbial community structures is mediated by the light-driven fluctuation of dominant cyanobacterial populations, and the diurnal variation patterns of specific populations may vary with habitats and sampling timepoints. This research provides valuable insights into the microbial community structure within the Zhubi lagoon.
光照的昼夜循环对微生物有显著影响，这使得昼夜研究对于理解微生物群落至关重要。众所周知，渚碧礁拥有异常丰富的生物多样性，浮游动物和海水属性都呈现出昼夜模式。然而，微生物群落结构及其潜在的昼夜动态在很大程度上仍未得到探索。本研究首次利用流式细胞术和高通量测序来研究渚碧泻湖中的原核生物和微型真核生物群落，重点关注不同光照强度下的昼夜变化。微微型浮游植物细胞丰度和微生物群落结构均呈现出明显的昼夜变化。光照被确定为微微型浮游植物细胞丰度昼夜变化的主要驱动因素。微生物群落多样性的昼夜变化是由两个主要的蓝细菌类群的细胞丰度变化驱动的。我们的研究结果表明，微生物群落结构的昼夜变化是由优势蓝细菌种群的光驱动波动介导的，特定种群的昼夜变化模式可能因栖息地和采样时间点而异。这项研究为渚碧泻湖内的微生物群落结构提供了有价值的见解。
