Song Zhenzhen, Zhang Ting, Liang Yantao, Mcminn Andrew, Wang Min, Jiao Nianzhi, Luo Tingwei
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Fujian Key Laboratory of Marine Carbon Sequestration, Carbon Neutral Innovation Research Center, Xiamen University, Xiamen 361102, China.
Microorganisms. 2025 Mar 27;13(4):764. doi: 10.3390/microorganisms13040764.
plays a pivotal role in the marine biogeochemical cycle. Advances in isolation techniques and high-throughput sequencing have expanded our understanding of the diversity of the community. However, their genomic diversity, functional dynamics and seasonal variations in the coastal waters are still not well known. Here, seawater samples were collected seasonally (March, June, August, December) from three stations in the coastal waters of Xiamen. Using fluorescence-activated cell sorting (FACS), we isolated 1000 cells per sample and performed ITS amplicon sequencing and metagenomic sequencing to analyze the seasonal variations in community structure and functional genes of . Firstly, we conducted a comparative analysis of in situ data and FACS data from three sampling sites in August. FACS samples revealed low-abundance strains underdetected by in situ samples. In addition, 24 clades representing subclusters S5.1, S5.2, and S5.3 were detected from three in situ samples and twelve FACS samples, suggesting the high diversity of in the coastal waters of Xiamen. Furthermore, the community displayed pronounced seasonal variations, and temperature significantly influenced the variations in community composition. Additionally, populations exhibit seasonal functional dynamics, with enhanced metabolic activity in summer characterized by higher numbers of functional genes associated with metabolic pathways compared to winter samples. Altogether, this study underscored the significance of FACS and high-throughput sequencing to reveal the diversity and functional dynamics of .
在海洋生物地球化学循环中起着关键作用。分离技术和高通量测序的进展扩大了我们对该群落多样性的认识。然而，它们在沿海水域的基因组多样性、功能动态和季节变化仍然不太清楚。在这里，季节性地（3月、6月、8月、12月）从厦门沿海水域的三个站点采集海水样本。使用荧光激活细胞分选技术（FACS），我们每个样本分离出1000个细胞，并进行ITS扩增子测序和宏基因组测序，以分析该群落结构和功能基因的季节变化。首先，我们对8月三个采样点的原位数据和FACS数据进行了比较分析。FACS样本揭示了原位样本未检测到的低丰度菌株。此外，从三个原位样本和十二个FACS样本中检测到代表亚簇S5.1、S5.2和S5.3的24个进化枝，表明厦门沿海水域中该生物的高度多样性。此外，该群落表现出明显的季节变化，温度显著影响该群落组成的变化。此外，该生物群体表现出季节性功能动态，与冬季样本相比，夏季代谢活动增强，其特征是与代谢途径相关的功能基因数量更多。总之，这项研究强调了FACS和高通量测序对于揭示该生物的多样性和功能动态的重要性。
