Knecht George T, Nauth Stephanie D, Alvarado Juan C Gomez, Santana Anthony M, Goicoechea Hector C, Campiglia Andres D
Department of Chemistry, University of Central Florida, Physical Sciences Building, 4111, Orlando, FL 32816, USA.
Laboratorio de Desarrollo Analítico y Quimiometría, Catedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Santa Fe 3000, Argentina.
Molecules. 2025 Mar 31;30(7):1550. doi: 10.3390/molecules30071550.
When high-performance liquid chromatography (HPLC) is used for the analysis of polycyclic aromatic hydrocarbons (PAHs) in complex samples, further examination of HPLC fractions is recommended to confirm PAH assignments solely based on retention times. Gas chromatography-mass spectrometry (GC-MS) has been particularly relevant in the unambiguous determination of PAHs with remarkably similar retention times. The combination of HPLC and GC requires lengthy analysis times to ensure proper assignments. This article presents an approach for the analysis of co-eluted PAHs with no need for further chromatographic separation. Benzo[a]pyrene (BaP) and dibenzo[a,l]pyrene (DBalP) were directly determined in a co-eluted HPLC fraction via room-temperature fluorescence excitation-emission matrices (RTF-EEMs). RTF-EEMs can be recorded in a matter of seconds with a spectrofluorometer equipped with a multichannel detection system. The spectral overlapping of BaP and DBalP was resolved using parallel factor analysis (PARAFAC). The analytical advantages of this approach were demonstrated with the trace analysis (ng/mL) of these two PAHs in pre-concentrated tobacco extracts.
当使用高效液相色谱法（HPLC）分析复杂样品中的多环芳烃（PAHs）时，建议对HPLC馏分进行进一步检测，以仅基于保留时间来确认PAH的归属。气相色谱-质谱联用（GC-MS）在明确测定保留时间极为相似的PAHs方面尤为重要。HPLC和GC的联用需要较长的分析时间以确保正确归属。本文介绍了一种无需进一步色谱分离即可分析共洗脱PAHs的方法。通过室温荧光激发-发射矩阵（RTF-EEMs）直接在共洗脱的HPLC馏分中测定苯并[a]芘（BaP）和二苯并[a,l]芘（DBalP）。使用配备多通道检测系统的荧光分光光度计可在数秒内记录RTF-EEMs。利用平行因子分析（PARAFAC）解决了BaP和DBalP的光谱重叠问题。通过对预浓缩烟草提取物中这两种PAHs的痕量分析（ng/mL）证明了该方法的分析优势。
