Piechowiak Tomasz, Balawejder Maciej
Department of Chemistry and Food Toxicology, Faculty of Technology and Life Sciences, University of Rzeszów, St. Ćwiklińskiej 1a, Rzeszów 35-601, Poland.
Department of Chemistry and Food Toxicology, Faculty of Technology and Life Sciences, University of Rzeszów, St. Ćwiklińskiej 1a, Rzeszów 35-601, Poland.
J Biotechnol. 2025 Aug;404:144-151. doi: 10.1016/j.jbiotec.2025.04.017. Epub 2025 Apr 24.
Succinic acid is a molecule with strong biological activity, whose primary role in eucaryotic cells is to participate in the Krebs cycle and maintain normal energy metabolism. In the present study, we aimed to test whether the application of succinic acid into radish sprout cultures would stimulate antioxidant production by improving the energy metabolism. Our results demonstrated that succinic acid enhanced mitochondrial activity by upregulating the expression of TCA enzymes and oxidative phosphorylation pathway. This leads to both increased biosynthesis of ATP and elevated production of reactive oxygen species (ROS). The ROS generated subsequently activate cellular defense mechanism against oxidative stress, resulting in upregulated expression of antioxidant enzymes and enhanced biosynthesis of antioxidants. However, the effect of succinate on antioxidant levels was dependent on its concentration in the water. The highest concentration (10 mM) resulted in sprouts with the greatest levels of ascorbate and glutathione, whereas the lowest concentration (0.1 mM) stimulated the production of phenolic compounds.
琥珀酸是一种具有强大生物活性的分子，其在真核细胞中的主要作用是参与三羧酸循环并维持正常的能量代谢。在本研究中，我们旨在测试将琥珀酸应用于萝卜芽苗培养物中是否会通过改善能量代谢来刺激抗氧化剂的产生。我们的结果表明，琥珀酸通过上调三羧酸循环（TCA）酶和氧化磷酸化途径的表达来增强线粒体活性。这导致ATP生物合成增加以及活性氧（ROS）生成增多。随后产生的ROS激活细胞针对氧化应激的防御机制，导致抗氧化酶表达上调和抗氧化剂生物合成增强。然而，琥珀酸盐对抗氧化水平的影响取决于其在水中的浓度。最高浓度（10 mM）使芽苗中的抗坏血酸和谷胱甘肽水平最高，而最低浓度（0.1 mM）则刺激了酚类化合物的产生。
