外源褪黑素调节干旱胁迫下葡萄柚薄荷的生理反应、植物化学特征和精油产量。

Exogenous melatonin modulates physiological responses, phytochemical profiles and essential oil production in grapefruit mint under drought stress.

作者信息

Rezaei Sara, Javanmard Abdollah, Sabaghnia Naser, Morshedloo Mohammad Reza

机构信息

Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, P.O. Box 55187-79842, Maragheh, Iran.

Department of Horticultural Science, Faculty of Agriculture, University of Maragheh, Maragheh, Iran.

出版信息

Sci Rep. 2025 Apr 26;15(1):14650. doi: 10.1038/s41598-025-99281-5.

Currently, the use of anti-stress elicitors such as melatonin, N-acetyl-5-methoxytryptamine, is known as an effective method to reduce the negative impacts of stressful environments. Therefore, a two factor- five level (irrigation levels: 25-75% field capacity (FC) and melatonin: 0-300 mM) dry yield optimization and modeling were done using central composite design, essential oil quantity and quality, total phenol, flavonoid and chlorophyll content and also some physiological properties of grapefruit mint (Mentha suaveolens × Mentha piperita). The results showed that the grapefruit mint biomass in severe drought stress was reduced by 73% in comparison with normal irrigation. However, the application of 155 mM melatonin enhanced biomass yield, phenol, flavonoid, essential oil content and essential oil yield by 92.2, 33.4, 55.1, 45.3 and 143%, in comparison with control. Optimization analysis predicted that under FC 65% irrigation level and application of 240 mM melatonin would positively assist in achieving the maximum chlorophyll, phenol, flavonoid content and essential oil yield of grapefruit mint. In addition, the maximum content of linalool and linalool acetate was observed in FC 50% treated with 155 mM melatonin. Generally, it can be concluded that the application of melatonin, especially in 155 mM level, improved plant growth, essential oil quantity and quality and also plant physiological characteristics under drought stress.

目前，使用褪黑素（N - 乙酰 - 5 - 甲氧基色胺）等抗应激诱导剂是减轻应激环境负面影响的有效方法。因此，采用中心复合设计对两个因素（灌溉水平：田间持水量的25 - 75%和褪黑素：0 - 300 mM）进行了干产量优化和建模，研究了葡萄柚薄荷（留兰香×胡椒薄荷）的精油数量和质量、总酚、黄酮和叶绿素含量以及一些生理特性。结果表明，与正常灌溉相比，严重干旱胁迫下葡萄柚薄荷的生物量降低了73%。然而，与对照相比，施用155 mM褪黑素使生物量产量、酚类、黄酮类、精油含量和精油产量分别提高了92.2%、33.4%、55.1%、45.3%和143%。优化分析预测，在田间持水量65%的灌溉水平和施用240 mM褪黑素的条件下，将有助于实现葡萄柚薄荷叶绿素、酚类、黄酮类含量和精油产量的最大化。此外，在155 mM褪黑素处理的田间持水量50%条件下，芳樟醇和乙酸芳樟酯的含量最高。总体而言，可以得出结论，褪黑素的施用，尤其是155 mM水平，在干旱胁迫下改善了植物生长、精油数量和质量以及植物生理特性。