Gao Ranran, Wu Yeke, Tang Yuqin, Wu Keming, Guo Baojun
Department of TCM, Henan Provincial People's Hospital, Zhengzhou University, No. 7 Weiwu Road, Jinshui District, Zhengzhou, Henan, 450003, China.
Department of Stomatology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 610072, China.
J Ovarian Res. 2025 Apr 26;18(1):87. doi: 10.1186/s13048-025-01679-2.
Premature ovarian failure (POF) is defined as amenorrhea that occurs before the age of 40 when the ovaries weaken or even fail. This disease seriously affects a woman's future health and fertility.
Potential targets of Yijing Decoction (YJD) and POF were predicted by web-based pharmacology-related databases. The POF rat models and human ovarian granulosa cells injury models were induced by triptolide. In addition, the estrous cycle of the rats was monitored by vaginal smear and the ovarian tissue morphology was stained by HE staining. Immunohistochemistry, qRT-PCR and Western blotting were used to evaluate the levels of reproductive and angiogenesis related factors. Moreover, serum levels of the sex hormones and the oxidative stress indicators were measured by ELISA.
YJD treatment resulted in the improvement of triptolide-induced abnormal ovarian function by restoring normal estrous cycle, maintaining nearly normal ovarian size, reducing follicular atresia and increasing vascularization. Additionally, YJD treatment normalized the serum levels of P, E2, FSH, LH, AMH, MDA and SOD, while activating the VEGF/VEGFR-2/FAK pathway. However, the VEGF/VEGFR-2/FAK pathway inhibitors reversed these pharmacological effects that YJD exhibited in POF rats. Furthermore, YJD increased the pregnancy rate and the number of live births in POF rats.
YJD reduced oxidative stress level, promoted angiogenesis and improved ovarian function in POF rats by activating VEGF/VEGFR-2/FAK pathway. Moreover, YJD improved the intrauterine microenvironment for implantation in POF rats, thereby improving fertility.
Not applicable.
卵巢早衰（POF）被定义为在40岁之前出现的闭经，此时卵巢功能减弱甚至衰竭。这种疾病严重影响女性未来的健康和生育能力。
通过基于网络的药理学相关数据库预测益经汤（YJD）和POF的潜在靶点。雷公藤甲素诱导建立POF大鼠模型和人卵巢颗粒细胞损伤模型。此外，通过阴道涂片监测大鼠的动情周期，并用苏木精-伊红（HE）染色观察卵巢组织形态。采用免疫组织化学、qRT-PCR和蛋白质免疫印迹法评估生殖和血管生成相关因子的水平。此外，通过酶联免疫吸附测定（ELISA）法检测血清性激素水平和氧化应激指标。
YJD治疗可恢复正常动情周期，维持卵巢大小接近正常，减少卵泡闭锁并增加血管生成，从而改善雷公藤甲素诱导的卵巢功能异常。此外，YJD治疗可使血清P、E2、FSH、LH、AMH、MDA和SOD水平恢复正常，同时激活VEGF/VEGFR-2/FAK通路。然而，VEGF/VEGFR-2/FAK通路抑制剂可逆转YJD在POF大鼠中表现出的这些药理作用。此外，YJD可提高POF大鼠的妊娠率和活产数。
YJD通过激活VEGF/VEGFR-2/FAK通路降低POF大鼠的氧化应激水平，促进血管生成并改善卵巢功能。此外，YJD改善了POF大鼠的子宫内植入微环境，从而提高生育能力。
不适用。
