用于定义啮齿动物新陈代谢的高功率、脑聚焦微波固定方案。
Protocol for high-power, brain-focused microwave fixation to define rodent metabolism.
作者信息
Cantrell Alex R, Hawkinson Tara R, Juras Jelena A, Webb Madison B, Medina Terrymar, Ribas Roberto A, Collins James, Bunnell Amelia A, Young Lyndsay E A, Markussen Kia H, Andres Douglas A, Long Joanna R, Vander Kooi Craig W, Smith Bret N, Sun Ramon C, Gentry Matthew S
机构信息
Department of Biochemistry and Molecular Biology, University of Florida, College of Medicine, Gainesville, FL 32610, USA.
Department of Molecular and Cellular Biochemistry, University of Kentucky, College of Medicine, Lexington, KY 40536, USA.
出版信息
STAR Protoc. 2025 Apr 25;6(2):103794. doi: 10.1016/j.xpro.2025.103794.
Analysis of metabolites provides key insights into brain physiology and function. Due to post-mortem metabolism, both the euthanasia method and dissection time can make a critical difference. Here, we describe a protocol to euthanize rodents by microwave irradiation. This workflow details steps for animal placement, tissue fixation, and post-fixation processing. This protocol enables the rapid halting of metabolic activity for the accurate assessment of the metabolome in situ for analyses such as mass spectrometry and nuclear magnetic resonance. For complete details on the use and execution of this protocol, please refer to Juras et al..
代谢物分析为深入了解大脑生理和功能提供了关键见解。由于死后代谢，安乐死方法和解剖时间都可能产生至关重要的影响。在此，我们描述一种通过微波辐射对啮齿动物实施安乐死的方案。该工作流程详细说明了动物安置、组织固定和固定后处理的步骤。此方案能够迅速停止代谢活动，以便在原位准确评估代谢组，用于质谱分析和核磁共振等分析。有关此方案使用和执行的完整详细信息，请参阅尤拉斯等人的研究。
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