Xiang Siyu, Yang Dong Kwon
Department of Veterinary Pharmacology & Toxicology, College of Veterinary Medicine, Jeonbuk National University, Specialized Campus, 79 Gobong-ro, Iksan 54596, Jeollabuk-do, Republic of Korea.
Biosafety Research Institute and Laboratory of Pathology, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Jeollabuk-do, Republic of Korea.
Nutrients. 2025 Mar 10;17(6):966. doi: 10.3390/nu17060966.
BACKGROUND/OBJECTIVES: The extract from aerial yam bulbils (AYB) contains various bioactive compounds, yet the mechanisms underlying its effects on APAP-induced liver injury need to be investigated further. This study sought to pursue the effects of AYB extract and the potential mechanisms involved in mitigating APAP-induced hepatotoxicity.
TIB-73 cells were pretreated with AYB extract (10, 20, and 40 μg/mL) for 24 h and treated with APAP for 24 h to induce cytotoxicity.
Analysis of apoptosis-related proteins revealed that AYB extract exerts anti-apoptotic effects and inhibiting the MAPK signaling pathways, thereby reducing apoptotic cell death. Additionally, AYB extract significantly suppressed ROS overproduction by enhancing the expression of endogenous antioxidants and reducing the endoplasmic reticulum (ER) stress in APAP-treated cells, indicating that AYB extract inhibits APAP-induced oxidative stress. AYB extract effectively preserved mitochondrial membrane potential (MMP), maintained mitochondrial function-related genes, reduced mitochondrial oxidative stress, and mitigated mitochondrial damage, thereby preserving mitochondrial integrity. Additionally, AYB extract activated the Nrf2-related signaling pathway through nuclear translocation, leading to the upregulation of downstream antioxidative target genes. Diosgenin, a compound with known antioxidant properties and hepatoprotective effects, was identified in significant quantities in the AYB extract, suggesting that it may contribute to the observed hepatoprotective effects.
Overall, these findings demonstrate that AYB extract, with its antioxidative properties, effectively protects TIB-73 cells from APAP-induced liver injury.
背景/目的：薯蓣珠芽提取物（AYB）含有多种生物活性化合物，但其对乙酰氨基酚（APAP）诱导的肝损伤的作用机制尚需进一步研究。本研究旨在探讨AYB提取物的作用及其减轻APAP诱导的肝毒性的潜在机制。
将TIB-73细胞用AYB提取物（10、20和40μg/mL）预处理24小时，然后用APAP处理24小时以诱导细胞毒性。
对凋亡相关蛋白的分析表明，AYB提取物发挥抗凋亡作用并抑制丝裂原活化蛋白激酶（MAPK）信号通路，从而减少凋亡细胞死亡。此外，AYB提取物通过增强内源性抗氧化剂的表达并减轻APAP处理细胞中的内质网（ER）应激，显著抑制活性氧（ROS）的过量产生，表明AYB提取物抑制APAP诱导的氧化应激。AYB提取物有效地维持线粒体膜电位（MMP），维持线粒体功能相关基因，减少线粒体氧化应激，并减轻线粒体损伤，从而保持线粒体完整性。此外，AYB提取物通过核转位激活Nrf2相关信号通路，导致下游抗氧化靶基因的上调。在AYB提取物中大量鉴定出具有已知抗氧化特性和肝保护作用的化合物薯蓣皂苷元，表明它可能有助于观察到的肝保护作用。
总体而言，这些发现表明，具有抗氧化特性的AYB提取物可有效保护TIB-73细胞免受APAP诱导的肝损伤。
