Mauro Manuela, Villanova Valeria, Valvo Mario Lo, Alduina Rosa, Radovic Slobodanka, Vizzini Aiti, Orecchio Grazia, Longo Francesco, Badalamenti Rosario, Ferrantelli Vincenzo, Cammilleri Gaetano, Mauro Annamaria, Arizza Vincenzo, Vazzana Mirella
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF) University of Palermo Palermo Italy.
NBFC, National Biodiversity Future Center Palermo Italy.
Ecol Evol. 2025 Apr 24;15(4):e71276. doi: 10.1002/ece3.71276. eCollection 2025 Apr.
Freshwater ecosystems play a key role in biogeochemical cycles and face various anthropogenic impacts. Understanding microbial communities within these ecosystems is crucial for ecosystem management and conservation. This study conducted a metabarcoding analysis of the bacterial 16S rDNA of environmental DNA (eDNA) to investigate the microbial diversity and composition in three Sicilian lakes (Poma, Piana, and Scanzano). The results revealed a common core microbiota in all the lakes, with Poma and Piana showing similar compositions, followed by Scanzano. Key bacterial phyla identified as core members across the sampled sites included Actinobacteriota, Proteobacteria, Bacteroidota, Verrucomicrobiota, Planctomycetota, and Cyanobacteria. Additionally, specific bacterial families such as Comamonadaceae, Burkholderiaceae, Ilumatobacteraceae, and Sporichthyaceae uniquely contributed to the microbial community structures. eDNA demonstrates potential as a quicker and less invasive tool to investigate biodiversity studies for these sites. The findings illuminate the intricate microbial dynamics within Sicilian freshwater ecosystems and emphasize the importance of considering microbial diversity in freshwater conservation and management strategies.
淡水生态系统在生物地球化学循环中发挥着关键作用，并面临着各种人为影响。了解这些生态系统中的微生物群落对于生态系统管理和保护至关重要。本研究对环境DNA（eDNA）的细菌16S rDNA进行了宏条形码分析，以调查西西里岛三个湖泊（波马湖、皮亚纳湖和斯坎扎诺湖）中的微生物多样性和组成。结果显示，所有湖泊中都存在一个共同的核心微生物群，波马湖和皮亚纳湖的组成相似，其次是斯坎扎诺湖。在所有采样点被确定为核心成员的关键细菌门包括放线菌门、变形菌门、拟杆菌门、疣微菌门、浮霉菌门和蓝细菌门。此外，一些特定的细菌科，如丛毛单胞菌科、伯克霍尔德菌科、发光杆菌科和芽孢鱼菌科，对微生物群落结构有独特贡献。eDNA显示出作为一种更快且侵入性更小的工具来研究这些地点生物多样性的潜力。这些发现揭示了西西里岛淡水生态系统中复杂的微生物动态，并强调了在淡水保护和管理策略中考虑微生物多样性的重要性。
