Sulieman Abdel Moneim E, Al-Azmi Meshari, Alanazi Naimah Asid, Ghoniem Ahmed Eisa, Hasan Mohamed El-Sayed, Elnahal Ahmed Said Mohamed, Alothman Norah S, Alrashidi Ayshah
Department of Biology, College of Science, University of Ha'il, Ha'il, Saudi Arabia.
College of Computer Science and Engineering, University of Ha'il, Ha'il, Saudi Arabia.
Front Microbiol. 2025 Apr 11;15:1500029. doi: 10.3389/fmicb.2024.1500029. eCollection 2024.
() and other plant infections threaten global agriculture and food security. This research incorporated strains in microbial consortia to boost plant tolerance to . The fungus causes collapse and deterioration in many crops like potatoes by quickly spreading through their tubers and leaves in warm, damp weather.
The main goals were to identify effective strains (those with high inhibitory activity), test their interactions (both inhibitory and synergistic), and determine the effect of inoculum density on disease treatment.
We used the following methodologies, from potato shoots and rhizosphere samples, Nine different strains of the antifungal bacterium which were identified with preliminary antifungal activity. Bintje showed the greatest resistance to among the three potato types that were examined. Methods utilized comprised: Quantification of bacterial density and growth, the inhibitory assays for , experiments on leaf disc infections, Assessing the severity of an infection, Analysis of zoospore discharge. Studies on the integrated development of bacteria and valuation using statistical methods.
The study revealed the complexity of microbial interactions, host-specific reactions, and cell density's impact on treatment success. The study suggests using strains as biocontrol agents, advancing sustainable agriculture. Microbial consortia disease management requires advanced methodologies, according to the findings. Investigating long-term ecological impacts on soil health, microbial diversity, and crop yield sustainability; validating identified microbial consortia through field trials; evaluating scalability and economic viability; and researching genetic engineering for customized disease control are recommended.
Results suggest a shift from chemical pesticides to environmentally friendly plant disease control considering its ethical and regulatory implications. This study emphasizes the intricacy of microbial interactions and the need for informed biocontrol decisions. Their study also increases ecological knowledge and encourages innovative, sustainable worldwide agriculture.
（）和其他植物感染威胁着全球农业和粮食安全。本研究将菌株纳入微生物群落，以提高植物对（）的耐受性。这种真菌会在温暖潮湿的天气中迅速通过许多作物（如土豆）的块茎和叶子传播，导致作物枯萎和变质。
主要目标是鉴定有效的（）菌株（具有高抑制活性的菌株），测试它们之间的相互作用（抑制和协同作用），并确定接种密度对疾病治疗的影响。
我们采用了以下方法，从马铃薯茎尖和根际样本中，筛选出九种具有初步抗真菌活性的抗真菌细菌菌株。在所检测的三种马铃薯品种中，宾杰对（）的抗性最强。所采用的方法包括：细菌密度和生长的定量分析、（）的抑制试验、叶盘感染实验、感染严重程度评估、游动孢子释放分析。对细菌的综合发育进行研究并采用统计方法进行评估。
该研究揭示了微生物相互作用、宿主特异性反应以及细胞密度对治疗成功的影响的复杂性。该研究建议将（）菌株用作生物防治剂，推动可持续农业发展。根据研究结果，微生物群落疾病管理需要先进的方法。建议研究对土壤健康、微生物多样性和作物产量可持续性的长期生态影响；通过田间试验验证已鉴定的微生物群落；评估可扩展性和经济可行性；以及研究用于定制疾病控制的基因工程。
结果表明，考虑到其伦理和监管影响，应从化学农药转向环境友好型植物病害控制。本研究强调了微生物相互作用的复杂性以及做出明智的生物防治决策的必要性。他们的研究还增加了生态知识，并鼓励创新的、可持续的全球农业。
