Dhasmana Archna, Dobhal Pooja, Sati Abhilekh, Santhanam Ayushi, Preetam Subham, Malik Sumira, Joshi Nupur, Rustagi Sarvesh, Deshwal Ravi K
Himalayan School of Biosciences, Swami Rama Himalayan University 248104 Dehradun India
Daegu Gyeongbuk Institute of Science and Technology (DGIST) Daegu 42988 Republic of Korea
RSC Adv. 2025 Apr 25;15(17):13300-13312. doi: 10.1039/d5ra01349f. eCollection 2025 Apr 22.
Long valued for their therapeutic qualities, shiitake mushrooms () are a staple of traditional Asian medicine and cuisine. They are high in bio-actives such as polysaccharides, proteins, lipids, vitamins, minerals, sterols, and phenolic compounds, which exhibit immunomodulatory, anticancer, antibacterial, anti-inflammatory, and anti-oxidant properties. Despite these advantages, the limited bioavailability and stability of shiitake's bio-active components often restrict their therapeutic use. Recent advances in nanotechnology have led to the development of nanoemulsions to encapsulate bioactives, which enhanced their bioavailability, stability, and therapeutic efficacy. In this study, we developed a biopolymeric blend of zein and chitosan as a nanoemulsion for the encapsulation of crude shiitake extract. Focusing on the synthesis and refinement of bio-compatible nanoemulsion formulations, this study investigates the medicinal potential of shiitake mushrooms and their nanoemulsions using several assays: the DPPH assay for anti-oxidant activity; the BSA denaturation assay for anti-inflammatory activity; the MIC test for antimicrobial activity; and the MTT assay for anticancer activity. This study aimed to attain three main goals: synthesis of nanoemulsions, biochemical analysis of shiitake extracts, and characterization of the therapeutic efficacy of the resulting formulations. This study found that shiitake nanoemulsions showed significantly improved bio-availability and therapeutic efficacy, suggesting promising applications in pharmaceuticals, nutraceuticals, cosmetics, medicine, and the food industry.
香菇长期以来因其治疗功效而受到重视，是传统亚洲医学和美食的主要食材。它们富含生物活性物质，如多糖、蛋白质、脂质、维生素、矿物质、固醇和酚类化合物，这些物质具有免疫调节、抗癌、抗菌、抗炎和抗氧化特性。尽管有这些优点，但香菇生物活性成分的生物利用度和稳定性有限，常常限制了它们的治疗用途。纳米技术的最新进展导致了用于封装生物活性物质的纳米乳液的开发，这提高了它们的生物利用度、稳定性和治疗效果。在本研究中，我们开发了一种由玉米醇溶蛋白和壳聚糖组成的生物聚合物混合物作为纳米乳液，用于封装香菇粗提物。本研究聚焦于生物相容性纳米乳液配方的合成与优化，使用多种测定方法研究香菇及其纳米乳液的药用潜力：用DPPH测定法检测抗氧化活性；用牛血清白蛋白变性测定法检测抗炎活性；用MIC试验检测抗菌活性；用MTT测定法检测抗癌活性。本研究旨在实现三个主要目标：纳米乳液的合成、香菇提取物的生化分析以及所得配方治疗效果的表征。本研究发现，香菇纳米乳液的生物利用度和治疗效果显著提高，表明在制药、营养保健品、化妆品、医学和食品工业中有广阔的应用前景。
