Yu Huibin, Ke Hongqin, Chen Tu, Huang Jia, Zhou Lei, Wang Shenqi
Department of Pharmacy, Renmin Hospital, Hubei University of Medicine, Shiyan 442000, China.
School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
ACS Omega. 2025 Apr 9;10(15):15393-15399. doi: 10.1021/acsomega.4c11595. eCollection 2025 Apr 22.
This study aimed to develop a novel adsorbent, Zn -loaded hexanediamine-chitosan (HDA-CS) beads, for the selective removal of human testosterone from plasma. The preparation involved synthesizing chitosan beads, modifying them with hexanediamine to enhance adsorption capacity and subsequently loading them with zinc ions. The effects of the HDA spacer, Zn concentration, and adsorption time on the adsorption percentage for testosterone were systematically investigated through a series of adsorption experiments. Results indicated that the Zn -HDA-CS beads achieved a maximum adsorption capacity of approximately 35% within 90 min. The analysis of plasma concentrations of Zn , albumin, and sex hormone-binding globulin (SHBG) indicated that the adsorption process involved a complex interaction between testosterone-bound SHBG and albumin for Zn binding sites on the beads. Additionally, the adsorbent demonstrated good storage stability and selectivity for testosterone, with minimal impact on total protein content in plasma. These findings highlight the potential of Zn -HDA-CS beads as an effective therapeutic option for managing testosterone levels in clinical settings, particularly in patients undergoing androgen deprivation therapy for prostate cancer.
本研究旨在开发一种新型吸附剂——载锌己二胺 - 壳聚糖（HDA - CS）微球，用于从血浆中选择性去除人体睾酮。制备过程包括合成壳聚糖微球，用己二胺对其进行改性以提高吸附能力，随后负载锌离子。通过一系列吸附实验系统研究了HDA间隔基、锌浓度和吸附时间对睾酮吸附率的影响。结果表明，Zn - HDA - CS微球在90分钟内实现了约35%的最大吸附容量。对血浆中锌、白蛋白和性激素结合球蛋白（SHBG）浓度的分析表明，吸附过程涉及与睾酮结合的SHBG和白蛋白之间在微球上的锌结合位点的复杂相互作用。此外，该吸附剂对睾酮表现出良好的储存稳定性和选择性，对血浆中总蛋白含量的影响最小。这些发现凸显了Zn - HDA - CS微球作为临床环境中管理睾酮水平的有效治疗选择的潜力，特别是对于接受前列腺癌雄激素剥夺治疗的患者。
