Fujinaka L E, Hyde D M, Plopper C G, Tyler W S, Dungworth D L, Lollini L O
Exp Lung Res. 1985;8(2-3):167-90. doi: 10.3109/01902148509057520.
To quantitate the response of respiratory bronchiolar (RB) epithelium and peribronchiolar connective tissue (PCT) to chronic exposure to high ambient levels of ozone, two groups of 8 adult male bonnet monkeys each were subjected 8 h daily for one year to 0.64 ppm (UV standard) ozone or filtered air, respectively. Blocks of tissue selected throughout the lung and from first generation RBs following airway microdissection had the following significant exposure-related changes: 57% greater volume of RB in the lung, 27% smaller diameter of RB lumen, 179% thicker media and intima of peribronchiolar arterioles, 61% thicker RB epithelium, and 77% thicker PCT. The increase in thickness of the RB wall resulted primarily from an 84% increase in PCT, with the remainder from the epithelium. Estimates of cellular numerical density showed an 81% increase in cuboidal bronchiolar cells and an 87% decrease in type 1 pneumocytes in the exposed group. Cell volumes from serial section reconstruction showed significantly larger cuboidal bronchiolar (79%), ciliated (117%), and type 2 (66%) cells over controls. Significant PCT changes included more amorphous extracellular matrix (288%), neutrophils (1523%), and lymphocytes/plasma cells (307%). The number of fibroblasts and the volume of extracellular fibers were larger than control values by 44% and 31% in the exposed group, but these changes were not statistically significant. Centriacinar changes due to exposure to long-term, high ambient ozone in bonnet monkeys results in narrowing of respiratory bronchioles primarily by peribronchiolar inflammation (inflammatory cells, fibers, amorphous matrix) and secondarily through hyperplasia of cuboidal bronchiolar cells.
为了量化呼吸性细支气管（RB）上皮和细支气管周围结缔组织（PCT）对长期暴露于高环境水平臭氧的反应，将两组各8只成年雄性冠毛猕猴，分别每天8小时暴露于0.64 ppm（紫外线标准）臭氧或过滤空气中，持续一年。在气道显微解剖后，从整个肺以及第一代RB中选取的组织块出现了以下与暴露相关的显著变化：肺中RB的体积增大57%，RB管腔直径减小27%，细支气管周围小动脉的中膜和内膜增厚179%，RB上皮增厚61%，PCT增厚77%。RB壁厚度的增加主要源于PCT增加84%，其余来自上皮。细胞数密度估计显示，暴露组立方细支气管细胞增加81%，Ⅰ型肺细胞减少87%。通过连续切片重建得到的细胞体积显示，立方细支气管细胞（79%）、纤毛细胞（117%）和Ⅱ型细胞（66%）比对照组显著增大。PCT的显著变化包括更多的无定形细胞外基质（288%）、中性粒细胞（1523%）以及淋巴细胞/浆细胞（307%）。暴露组中成纤维细胞数量和细胞外纤维体积比对照值分别大44%和31%，但这些变化无统计学意义。帽猴长期暴露于高环境臭氧导致的腺泡中心变化，主要通过细支气管周围炎症（炎症细胞、纤维、无定形基质）导致呼吸性细支气管狭窄，其次是立方细支气管细胞增生。
