Seidel A, Heumann H G, Sütterlin U, Wiener M, Haffner H
Eur J Cell Biol. 1985 May;37:89-97.
The study was undertaken in order to understand the reasons for the distinct differences in the elimination rate of lanthanides and transuranium elements from the liver of different mammalian species. The binding of monomeric 239Pu in livers of rats and Chinese hamsters was analyzed by density gradient centrifugation and electrophoresis. It was concluded that this nuclide is initially bound to lysosomes in liver of rats and Chinese hamsters. The influence of Triton WR 1339 (TWR) on the density of lysosomal marker enzymes from rat and Chinese hamster liver at day 4 was very similar for both animal species but the TWR induced shift persisted in Chinese hamsters up to day 60 whereas in rat liver the lysosomal density increased again with time. Electron microscopic inspection confirmed the similarity of the initial reaction of hepatocyte lysosomes. However, after 60 to 70 days typical TWR induced "tritosomes" were absent from rat hepatocytes but could be found regularly in hepatocytes from Chinese hamsters. The elimination rate of 3H-activity from liver injection of 3H-TWR was lower in Chinese hamsters than in rats. It was concluded that the differences in elimination rate of lanthanides and transuranium elements from liver of various mammalian species and the differences observed after TWR injection might reflect differences in the composition or function of the lysosomal system in the livers of different mammalian species. With respect to the transport of certain heavy metals the rat liver is not a reliable model for human liver.
进行这项研究是为了了解不同哺乳动物肝脏中镧系元素和超铀元素消除率存在显著差异的原因。通过密度梯度离心和电泳分析了大鼠和中国仓鼠肝脏中单体239Pu的结合情况。得出的结论是，这种核素最初与大鼠和中国仓鼠肝脏中的溶酶体结合。在第4天，Triton WR 1339（TWR）对大鼠和中国仓鼠肝脏溶酶体标记酶密度的影响在两种动物中非常相似，但TWR诱导的变化在中国仓鼠中持续到第60天，而在大鼠肝脏中，溶酶体密度随时间再次增加。电子显微镜检查证实了肝细胞溶酶体初始反应的相似性。然而，60至70天后，大鼠肝细胞中没有典型的TWR诱导的“tritosomes”，但在中国仓鼠肝细胞中经常可以发现。中国仓鼠肝脏注射3H-TWR后3H活性的消除率低于大鼠。得出的结论是，不同哺乳动物肝脏中镧系元素和超铀元素消除率的差异以及注射TWR后观察到的差异可能反映了不同哺乳动物肝脏中溶酶体系统组成或功能的差异。就某些重金属的转运而言，大鼠肝脏不是人类肝脏的可靠模型。
