Yeoh G C, Brighton V J, Angus D A, Kraemer M, Vassy J, Chalemeau M T
Eur J Cell Biol. 1985 Jul;38(1):157-64.
Hepatocytes derived from 15 and 19-day gestation rats synthesize and secrete albumin during culture. Albumin secretion is maintained when the culture medium is supplemented with dexamethasone but declines in its absence. The fall in secretion rate correlates with the level of albumin messenger RNA in the respective cultures. Even when dexamethasone is present, the level of albumin production in 19-day gestation hepatocytes is 6 to 7 times greater than that observed in hepatocytes derived from 15-day gestation rats. Immunocytochemical studies were undertaken to establish whether the difference in secretion rate was due to a difference in the amount of albumin produced by all the hepatocytes of the respective cultures or whether there were fewer hepatocytes which were capable of synthesizing albumin in the less mature liver. The results indicate that albumin production is reduced in all hepatocytes when cultured in the absence of dexamethasone.
来自妊娠15天和19天大鼠的肝细胞在培养过程中合成并分泌白蛋白。当培养基中添加地塞米松时，白蛋白分泌得以维持，而在没有地塞米松的情况下则会下降。分泌率的下降与相应培养物中白蛋白信使核糖核酸的水平相关。即使存在地塞米松，妊娠19天肝细胞中的白蛋白产生水平也比来自妊娠15天大鼠的肝细胞中观察到的水平高6至7倍。进行了免疫细胞化学研究，以确定分泌率的差异是由于相应培养物中所有肝细胞产生的白蛋白量不同，还是在不太成熟的肝脏中能够合成白蛋白的肝细胞较少。结果表明，在没有地塞米松的情况下培养时，所有肝细胞中的白蛋白产生都会减少。
