一种评估发芽和未发芽卡拉 kratok（L.）中 HMG 辅酶 A 还原酶生物肽抑制剂生物利用度的新方法。

A novel approach to assessing the bioavailability of biopeptide inhibitor of HMG CoA reductase from germinated and ungerminated Kara Kratok ( L.).

作者信息

Budiyanto Cahyo, Ningrum Andriati, Murdiati Agnes, Indrati Retno

机构信息

Faculty of Agricultural Technology, Universitas Gadjah Mada, Department of Food and Agricultural Product Technology, Yogyakarta, Yogyakarta, Indonesia.

出版信息

PeerJ. 2025 Apr 23;13:e19262. doi: 10.7717/peerj.19262. eCollection 2025.

BACKGROUND

The bioavailability of biopeptide compounds is a development challenge, mainly because of their resistance to the digestion system. This study aimed to determine the bioavailability of HMG CoA reductase biopeptide inhibitors from germinated and ungerminated Kara Kratok ( L.).

METHODS

Germinated and ungerminated brown were simulated for digestion enzyme (120 minutes for pepsin and pancreatin), followed by an method for absorption. Perfusate samples were measured for the absorption percentage, inhibition of HMG CoA reductase, molecular weight (MW), peptide concentration, and hydrolysis degree (%DH).

RESULTS

The results showed that germinated brown exhibited the highest absorption (32.42%), and the percentage of HMG CoA reductase inhibition during enzymatic digestion was at 210 minutes (87.51%), with MW < 10 kDa, peptide concentration of 2.39 mg/mL, and %DH of 48.90%. These findings suggest that germinated brown is a potent HMG CoA reductase inhibitor with significantly higher bioavailability than that of its ungerminated counterpart. This finding underscores its superiority in this context and open new possibilities for biopeptide research.

背景

生物肽化合物的生物利用度是一个发展挑战，主要是因为它们对消化系统具有抗性。本研究旨在测定发芽和未发芽的卡拉 kratok（L.）中 HMG CoA 还原酶生物肽抑制剂的生物利用度。

方法

模拟发芽和未发芽的棕色种子进行消化酶处理（胃蛋白酶和胰蛋白酶处理 120 分钟），然后采用吸收方法。测定灌注液样品的吸收百分比、HMG CoA 还原酶抑制率、分子量（MW）、肽浓度和水解度（%DH）。

结果

结果表明，发芽的棕色种子表现出最高的吸收率（32.42%），酶消化过程中 HMG CoA 还原酶抑制率在 210 分钟时达到 87.51%，分子量<10 kDa，肽浓度为 2.39 mg/mL，水解度为 48.90%。这些发现表明，发芽的棕色种子是一种有效的 HMG CoA 还原酶抑制剂，其生物利用度明显高于未发芽的种子。这一发现突出了其在这方面的优越性，并为生物肽研究开辟了新的可能性。