Abalde-Pujales Alberto, Romero Vanesa, Lavilla Isela, Bendicho Carlos
Centro de Investigación Mariña, Universidade de Vigo, Departamento de Química Analítica y Alimentaria, Grupo QA2, 36310 Vigo, Spain.
Sensors (Basel). 2025 Mar 12;25(6):1756. doi: 10.3390/s25061756.
In this work, a novel 3D μPAD cellulose-based colorimetric chemosensor for multiplexed detection of paracetamol and aspirin in biological samples is proposed. The easy availability of analgesics such as paracetamol and non-steroidal anti-inflammatory drugs such as aspirin, over-the-counter drugs that can be acquired without medical prescription, can entail a health problem if they are administered incorrectly. The development of analytical procedures for the rapid, sensitive, and accurate determination of such drugs in clinical samples is of utmost importance. Different parameters involved in the design of the 3D μPAD system and the colorimetric reaction conditions have been optimized. Under optimal conditions, detection limits of 0.004 mM and 0.013 mM were obtained for paracetamol and aspirin, respectively. The proposed procedure was validated against two certified reference materials and applied to the analysis of several synthetic urine and saliva samples. Synthetic urine and saliva samples were spiked at two concentration levels, showing recoveries in the range of 98-103% with a relative standard deviation of 3-6% ( = 6).
在这项工作中，提出了一种新型的基于3D微流控纸基分析装置（μPAD）的纤维素比色化学传感器，用于生物样品中对乙酰氨基酚和阿司匹林的多重检测。像对乙酰氨基酚这样的镇痛药以及像阿司匹林这样的非甾体抗炎药很容易获得，它们是无需医生处方就能买到的非处方药，如果使用不当可能会引发健康问题。开发用于临床样品中此类药物快速、灵敏和准确测定的分析方法至关重要。对3D μPAD系统设计中涉及的不同参数以及比色反应条件进行了优化。在最佳条件下，对乙酰氨基酚和阿司匹林的检测限分别为0.004 mM和0.013 mM。所提出的方法针对两种有证标准物质进行了验证，并应用于几种合成尿液和唾液样品的分析。合成尿液和唾液样品在两个浓度水平加标，回收率在98 - 103%范围内，相对标准偏差为3 - 6%（n = 6）。
